RNA synthesis and number of transcribing polymerase molecules in ischemic liver nuclei.

Molecular and cellular biochemistry ·Vol. 48 ·No. 1 ·1982-10-01 ·Pages 59-63

Schiaffonati L, Cairo G, Bernelli-Zazzera A

Abstract

Nuclei isolated from ischemic liver show a consistent reduction of their RNA synthesis. The reduction persists at high ionic strength and in the presence of heparin, when RNA synthesis in vitro is fully activated and occurs in the presence as well as in the absence of alpha-amanitin. Both the number of transcribing polymerase molecules and the rate of elongation of initiated polynucleotide chains seem to be equally affected.

MeSH Terms

Animals Cell Nucleus/metabolism DNA-Directed RNA Polymerases/metabolism Ischemia/metabolism Kinetics Liver/blood supply,metabolism Male RNA/genetics Rats Rats, Inbred Strains Transcription, Genetic Uridine/metabolism Uridine Monophosphate/metabolism

Chemicals

RNA Uridine Monophosphate DNA-Directed RNA Polymerases Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schiaffonati L

Cairo G

Bernelli-Zazzera A

References (25)

25 references, click to expand

Decreased molecular weight of RNA transcribed following kidney storage.
Exp Mol Pathol. 1975 Feb;22(1):45-54 PMID: 1116566
Effect of amino acids on the alpha-amanitin-insensitive RNA polymerase activity in the isolated nuclei of Ehrlich ascites cells.
Biochim Biophys Acta. 1973 Nov 26;331(1):71-80 PMID: 4796862
Comparative effect of heparin on RNA synthesis of isolated rat-liver nucleoli and purified RNA polymerase A.
Eur J Biochem. 1975 May 6;53(2):605-13 PMID: 1095373
Diurnal rhythmicity of mammalian DNA-dependent RNA polymerase activities I and II: dependence on food intake.
Biochem Biophys Res Commun. 1973 Sep 5;54(1):62-8 PMID: 4795371
Pre-ribosomal RNA synthesis in isolated rat-liver nucleoli.
Eur J Biochem. 1973 Jul 2;36(1):244-9 PMID: 4738940
Regulation of the synthesis and the stability of ribosomal RNA during contact inhibition of growth.
Nat New Biol. 1971 Jul 28;232(30):101-6 PMID: 5284943
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Quantification of hepatic transcribing RNA polymerase molecules, polyribonucleotide elongation rates and messenger RNA complexity in fed and fasted rats.
Eur J Biochem. 1978 Mar 15;84(2):611-23 PMID: 639806
The mechanism by which heparin stimulates transcription in isolated rat liver nuclei. Polyribonucleotide elongation rates and the number of transcribing RNA polymerase molecules present.
Eur J Biochem. 1977 Oct 3;79(2):525-33 PMID: 923565
Rapid increase in poly(A) (+) mRNA content following inhibition of protein synthesis.
J Cell Physiol. 1977 Jul;92(1):57-64 PMID: 561079
Quantitation of elongating form A and B RNA polymerases in chick oviduct nuclei and effects of estradiol.
Cell. 1976 Mar;7(3):455-65 PMID: 181146
Pre-4S RNA made in isolated HeLa cell nuclei terminates with U.
Cell. 1974 Oct;3(2):185-8 PMID: 4426091
A procedure for the isolation of enzymically active rat-liver nuclei.
Biochem J. 1964 Aug;92(2):313-7 PMID: 4284460
Relationships between polyamine metabolism and RNA synthesis in post-ischemic liver cell repair.
J Cell Physiol. 1980 Apr;103(1):121-8 PMID: 6159362
The control of ribosomal RNA transcription in lymphocytes. Evidence that the rate of chain elongation is the limiting factor.
Eur J Biochem. 1981 Mar;114(3):487-92 PMID: 6165579
RNA metabolism in nuclei isolated from HeLa cells.
Eur J Biochem. 1975 Jun 16;55(1):61-70 PMID: 1175608
Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.
Cell. 1976 Mar;7(3):439-45 PMID: 947551
SOME ASPECTS OF GLYCOGEN METABOLISM FOLLOWING REVERSIBLE OR IRREVERSIBLE LIVER ISCHEMIA.
Exp Mol Pathol. 1964 Aug;3:351-68 PMID: 14204938
Effect of the ionic environment on the transcriptional activity of rat liver nucleoli.
Exp Cell Res. 1976 Oct 15;102(2):365-75 PMID: 976353
Initiation of DNA-dependent RNA synthesis and the effect of heparin on RNA polymerase.
Eur J Biochem. 1967 Dec;3(2):194-201 PMID: 4865568
Mechanism of aflatoxin B1 inhibition of rat hepatic nuclear RNA synthesis.
J Biol Chem. 1977 May 25;252(10):3245-51 PMID: 863881
The effects of histidine starvation on the methylation of ribosomal RNA.
Eur J Biochem. 1977 Sep 15;79(1):133-41 PMID: 913415
Cell repair after liver injury. Stimulation of RNA synthesis, engaged polymerases, number of RNA transcribing molecules, and elongation rate in postischemic liver nuclei.
Exp Mol Pathol. 1981 Oct;35(2):231-43 PMID: 6169542
Uterine ribonucleic acid polymerase. Effect of estrogen on nucleotide incorporation into 3' chain termini.
Biochemistry. 1971 Jun 8;10(12):2384-90 PMID: 5114997
RNA synthesis by nuclei and nucleoli from ischemic liver cells.
J Cell Physiol. 1978 Dec;97(3 Pt 2 Suppl 1):487-96 PMID: 83325

Article Info

Journal

Molecular and cellular biochemistry

Abbr.

Mol Cell Biochem

ISSN

0300-8177

DOI

10.1007/BF00214822

Published

1982-10-01

Pages

59-63

Language

English

Region

Netherlands

NLM ID

0364456

Subset

External Links

PubMed source DOI: 10.1007/BF00214822 View journal details

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RNA synthesis and number of transcribing polymerase molecules in ischemic liver nuclei.

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