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PMID: 2552411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vaccinia virus encodes a thymidylate kinase gene: sequence and transcriptional mapping.

Nucleic acids research ·Vol. 17 ·No. 19 ·1989-10-11 ·Pages 7581-90

Smith GL, de Carlos A, Chan YS

Abstract

The nucleotide sequence and deduced amino acid sequence of a vaccinia virus gene from the SalI F fragment are shown. The predicted polypeptide shares 42% amino acid identity over a 200 amino acid region with Saccharomyces cerevisiae thymidylate kinase (TmpK) and has low homology with herpes simplex virus deoxypyrimidine kinase. Northern blotting and S1 nuclease protection showed that the TmpK gene is transcribed early during infection and mapped the mRNA 5' end to immediately upstream of the second inframe ATG codon of the open reading frame (ORF). The encoded polypeptide is predicted to be 204 amino acids long (23.2 kD) and is almost colinear with yeast TmpK. Vaccinia virus possesses genes for TK and TmpK, separated by 57 kilobases of DNA, which are co-ordinately expressed and the encoded enzymes perform sequential steps in the same biochemical pathway.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cell Line Cloning, Molecular Genes, Viral Humans Molecular Sequence Data Nucleoside-Phosphate Kinase/genetics Phosphotransferases/genetics Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid Software Transcription, Genetic Vaccinia virus/enzymology,genetics Viral Structural Proteins/genetics
Chemicals
Viral Structural Proteins Phosphotransferases Nucleoside-Phosphate Kinase dTMP kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith G L
Department of Pathology, University of Cambridge, UK.
de Carlos A
Chan Y S
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.19.7581
Published
1989-10-11
Pages
7581-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334868
Subset
IM
Databases
GENBANK
X16259
External Links
PubMed source DOI: 10.1093/nar/17.19.7581 PMC Full Text View journal details
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