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PMID: 3025846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9566-70

Rodriguez JF, Kahn JS, Esteban M

Abstract

A rabbit poxvirus genomic library contained within the expression vector lambda gt11 was screened with polyclonal antiserum prepared against vaccinia virus nucleic acid-dependent nucleoside triphosphatase (NTPase)-I enzyme. Five positive phage clones containing from 0.72- to 2.5-kilobase-pair (kbp) inserts expressed a beta-galactosidase fusion protein that was reactive by immunoblotting with the NTPase-I antibody. Hybridization analysis allowed the location of this gene within the vaccinia HindIIID restriction fragment. From the known nucleotide sequence of the 16-kbp vaccinia HindIIID fragment, we identified a region that contains a 1896-base open reading frame coding for a 631-amino acid protein. Analysis of the complete sequence revealed a highly basic protein, with hydrophilic COOH and NH2 termini, various hydrophobic domains, and no significant homology to other known proteins. Translational studies demonstrate that NTPase-I belongs to a late class of viral genes. This protein is highly conserved among Orthopoxviruses.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Animals Base Sequence Chromosome Mapping Cloning, Molecular DNA, Viral/genetics Gene Expression Regulation Genes, Viral Nucleoside-Triphosphatase Phosphoric Monoester Hydrolases/genetics,immunology Rabbits Solubility Vaccinia virus/genetics
Chemicals
DNA, Viral Adenosine Triphosphate Phosphoric Monoester Hydrolases Nucleoside-Triphosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rodriguez J F
Kahn J S
Esteban M
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9566
Published
1986-12-00
Pages
9566-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387181
Subset
IM
Grants
NIAID NIH HHS · AI 16780 · United States
NCI NIH HHS · CA 44262 · United States
Databases
GENBANK
M14629
External Links
PubMed source DOI: 10.1073/pnas.83.24.9566 PMC Full Text View journal details
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