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PMID: 3550432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 7 ·No. 3 ·1987-03-00 ·Pages 1198-207

Rothstein R, Helms C, Rosenberg N

Abstract

Deletions of a tyrosine tRNA suppressor gene, SUP4-o, are mediated by recombination between short repeated delta sequences in Saccharomyces cerevisiae. The arrangement of the five solo delta sequences that surround the SUP4 locus was established by DNA sequence analysis. Seven deletion classes were identified by genomic blotting. DNA sequence analysis also showed that the delta sequences within a 6.5-kilobase region of the SUP4 locus were the endpoints of these events. In three of these classes, an adjacent interval surrounded by delta sequences was inverted in concert with the deletion. The frequency of all deletion classes decreased in strains that contained mutations in the recombination and repair gene RAD52. We present two gene conversion mechanisms by which these rearrangements could have been generated. These models may also explain deletions between repeated sequences in other systems.

MeSH Terms
Base Sequence Chromosome Deletion DNA, Fungal/genetics Gene Conversion Genes, Fungal Mitosis Models, Genetic Mutation RNA, Transfer, Amino Acyl/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics Suppression, Genetic
Chemicals
DNA, Fungal RNA, Transfer, Amino Acyl
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rothstein R
Helms C
Rosenberg N
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49 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.3.1198-1207.1987
Published
1987-03-00
Pages
1198-207
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365193
Subset
IM
Grants
NIGMS NIH HHS · GM-34587 · United States
Databases
GENBANK
M15430, M15431, M15468
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/mcb.7.3.1198-1207.1987 PMC Full Text View journal details
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