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PMID: 2835495 Published · ppublish English Journal Article

Sequence and transcriptional analysis of the vaccinia virus HindIII I fragment.

Journal of virology ·Vol. 62 ·No. 6 ·1988-06-00 ·Pages 1889-97

Schmitt JF, Stunnenberg HG

Abstract

The complete sequence of the vaccinia virus HindIII I fragment, which is composed of 6,498 base pairs, encodes six complete and two incomplete open reading frames (ORFs). Computer analysis revealed an amino acid sequence homology between ORF I 4 and the large subunit of the ribonucleotide reductase complex. The two small polypeptides derived from ORFs I 2 and I 5, with molecular weights of 8,500 and 8,700, respectively, have a very high hydrophobic amino acid sequence composition. S1 analysis revealed that ORF I 4 is expressed at early stages of infection, ORFs I 1, I 2, I 5, and I 7 are expressed in the late phase of infection, and ORF I 3 is constitutively expressed. Screening a vaccinia virus genomic library revealed a large vaccinia virus insert overlapping the HindIII I and O fragments which contains a previously undetected HindIII P fragment of approximately 300 base pairs. S1 analysis revealed an early (O1) and a late (O2) start site of transcription initiation located within the HindIII O fragment.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes/metabolism DNA, Viral/genetics Deoxyribonuclease HindIII Endonucleases Gene Expression Regulation Genes, Viral Molecular Sequence Data Promoter Regions, Genetic Protein Conformation Regulatory Sequences, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Terminator Regions, Genetic Transcription, Genetic Vaccinia virus/genetics Viral Proteins/genetics Virus Replication
Chemicals
DNA, Viral Viral Proteins Endonucleases DNA Restriction Enzymes Deoxyribonuclease HindIII Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schmitt J F
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Stunnenberg H G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.6.1889-1897.1988
Published
1988-06-00
Pages
1889-97
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253271
Subset
IM
Databases
GENBANK
J03399
External Links
PubMed source DOI: 10.1128/JVI.62.6.1889-1897.1988 PMC Full Text View journal details
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