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PMID: 25497060 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Selective autophagy: xenophagy.

Methods (San Diego, Calif.) ·Vol. 75 ·2015-03-00 ·Pages 120-7

Bauckman KA, Owusu-Boaitey N, Mysorekar IU

Abstract

Xenophagy is an autophagic phenomenon that specifically involves pathogens and other non-host entities. Although the understanding of the relationship between autophagosomes and invading organisms has grown significantly in the past decade, the exact steps to confirm xenophagy has been not been thoroughly defined. Here we describe a methodical approach to confirming autophagy, its interaction with bacterial invasion, as well as the specific type of autophagic formation (i.e. autophagosome, autolysosome, phagolysosome). Further, we argue that xenophagy is not limited to pathogen interaction with autophagosome, but also non-microbial entities such as iron.

Keywords
Ferritinophagy Infection Inflammasome LC3 Associated Phagocytosis Symbiophagy UPEC
MeSH Terms
Autophagy/genetics Brucella/genetics,pathogenicity Host-Pathogen Interactions/genetics Humans Infections/genetics,microbiology Lysosomes/metabolism Molecular Biology/methods Phagosomes/genetics,microbiology,pathology
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bauckman Kyle A
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO, USA.
Owusu-Boaitey Nana
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO, USA.
Mysorekar Indira U
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: indira@wustl.edu.
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Article Info
Journal
Methods (San Diego, Calif.)
Abbr.
Methods
ISSN
1095-9130
DOI
10.1016/j.ymeth.2014.12.005
Email
indira@wustl.edu
Published
2015-03-00
Epub
2014-00-11
Pages
120-7
Language
English
Region
United States
NLM ID
9426302
PMCID
PMC4355331
Subset
IM
Grants
NIDDK NIH HHS · R01 DK100644 · United States
NIAID NIH HHS · T32 AI007172 · United States
NICHD NIH HHS · T32 HD049305 · United States
NIAID NIH HHS · T32 AI 007172-35 · United States
External Links
PubMed source DOI: 10.1016/j.ymeth.2014.12.005 PMC Full Text View journal details
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