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PMID: 21997369 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Autophagy stimulation by rapamycin suppresses lung inflammation and infection by Burkholderia cenocepacia in a model of cystic fibrosis.

Autophagy ·Vol. 7 ·No. 11 ·2011-11-00 ·Pages 1359-70

Abdulrahman BA, Khweek AA, Akhter A, Caution K, Kotrange S, Abdelaziz DH, Newland C, Rosales-Reyes R, Kopp B, McCoy K, Montione R, Schlesinger LS, Gavrilin MA, Wewers MD, Valvano MA, Amer AO

Abstract

Cystic fibrosis (CF) is the most common inherited lethal disease of Caucasians which results in multi organ dysfunction. However, 85% of the deaths are due to pulmonary infections. Infection by Burkholderia cenocepacia (B. cepacia) is a particularly lethal threat to CF patients because it causes severe and persistent lung inflammation and is resistant to nearly all available antibiotics. In CFTR ΔF508 mouse macrophages, B. cepacia persists in vacuoles that do not fuse with the lysosomes and mediates increased production of IL-1β. It is believed that intracellular bacterial survival contributes to the persistence of the bacterium. Here we show for the first time that in wild-type macrophages but not in ΔF508 macrophages, many B. cepacia reside in autophagosomes that fuse with lysosomes at later stages of infection. Accordingly, association and intracellular survival of B. cepacia are higher in CFTR-ΔF508 (ΔF508) macrophages than in WT macrophages. An autophagosome is a compartment that engulfs non-functional organelles and parts of the cytoplasm then delivers them to the lysosome for degradation to produce nutrients during periods of starvation or stress. Furthermore, we show that B. cepacia downregulates autophagy genes in WT and ΔF508 macrophages. However, autophagy dysfunction is more pronounced in ΔF508 macrophages since they already have compromised autophagy activity. We demonstrate that the autophagy-stimulating agent, rapamycin markedly decreases B. cepacia infection in vitro by enhancing the clearance of B. cepacia via induced autophagy. In vivo, Rapamycin decreases bacterial burden in the lungs of CF mice and drastically reduces signs of lung inflammation. Together, our studies reveal that if efficiently activated, autophagy can control B. cepacia infection and ameliorate the associated inflammation. Therefore, autophagy is a novel target for new drug development for CF patients to control B. cepacia infection and accompanying inflammation.

MeSH Terms
Animals Autophagy/drug effects,genetics Burkholderia Infections/complications,drug therapy,microbiology,pathology Burkholderia cenocepacia/drug effects,growth & development,physiology,ultrastructure Cystic Fibrosis/complications,drug therapy,microbiology,pathology Cystic Fibrosis Transmembrane Conductance Regulator/metabolism Disease Models, Animal Down-Regulation/genetics Interleukin-1beta/biosynthesis Intracellular Space/drug effects,microbiology Lysosomes/drug effects,microbiology Macrophages/drug effects,metabolism,microbiology,ultrastructure Mice Mice, Inbred C57BL Microbial Viability/drug effects Microtubule-Associated Proteins/metabolism Mutation/genetics Phagosomes/drug effects,microbiology,ultrastructure Pneumonia/complications,drug therapy,microbiology RNA, Small Interfering/metabolism Sirolimus/pharmacology,therapeutic use Vacuoles/drug effects,microbiology
Chemicals
Interleukin-1beta Map1lc3b protein, mouse Microtubule-Associated Proteins RNA, Small Interfering cystic fibrosis transmembrane conductance regulator delta F508 Cystic Fibrosis Transmembrane Conductance Regulator Sirolimus
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
Abdulrahman Basant A
Center for Microbial Interface Biology, Department of Microbial Infection, Ohio State University, Columbus, OH, USA.
Khweek Arwa Abu
Akhter Anwari
Caution Kyle
Kotrange Sheetal
Abdelaziz Dalia H A
Newland Christie
Rosales-Reyes Roberto
Kopp Benjamin
McCoy Karen
Montione Richard
Schlesinger Larry S
Gavrilin Mikhail A
Wewers Mark D
Valvano Miguel A
Amer Amal O
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Article Info
Journal
Autophagy
Abbr.
Autophagy
ISSN
1554-8635
Published
2011-11-00
Epub
2011-00-01
Pages
1359-70
Language
English
Region
United States
NLM ID
101265188
PMCID
PMC3359483
Subset
IM
Grants
NIDDK NIH HHS · P30 DK027651 · United States
PHS HHS · R21AL083871 · United States
NHLBI NIH HHS · R01 HL094586 · United States
NHLBI NIH HHS · R01 HL094586-03 · United States
NHLBI NIH HHS · R01HL094586 · United States
NHLBI NIH HHS · R01 HL094586-02 · United States
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