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PMID: 2546921 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic characterization of the pnuC gene, which encodes a component of the nicotinamide mononucleotide transport system in Salmonella typhimurium.

Journal of bacteriology ·Vol. 171 ·No. 8 ·1989-08-00 ·Pages 4402-9

Zhu N, Olivera BM, Roth JR

Abstract

The pnuC gene, which encodes a component of the nicotinamide mononucleotide transport system, has been mapped and oriented. The gene order of the pnuC region, which is at min 17 of the Salmonella chromosome, is nadA-pnuC-aroG-gal. Polarity tests, with pnuC::Mu d-lac operon fusions, reveal that the pnuC gene is the promoter distal gene in an operon with the nadA gene, which encodes the second enzyme of the pyridine biosynthetic pathway. The nadA pnuC operon is regulated by the NadI repressor. The pnuC gene also has its own promoter, since strains with a nadA::Tn10d(Tc) insertion still express the pnuC gene at a low, unregulated level.

MeSH Terms
Bacterial Proteins/genetics Biological Transport Chromosome Deletion DNA Transposable Elements Genes Genes, Bacterial Genotype Mutation Nicotinamide Mononucleotide/metabolism Recombination, Genetic Salmonella typhimurium/genetics,metabolism Transduction, Genetic
Chemicals
Bacterial Proteins DNA Transposable Elements Nicotinamide Mononucleotide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhu N
Department of Biology, University of Utah, Salt Lake City 84112.
Olivera B M
Roth J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.8.4402-4409.1989
Published
1989-08-00
Pages
4402-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210218
Subset
IM
Grants
NIGMS NIH HHS · GM23408 · United States
NIGMS NIH HHS · GM25654 · United States
External Links
PubMed source DOI: 10.1128/jb.171.8.4402-4409.1989 PMC Full Text View journal details
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