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PMID: 3275606 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a repressor gene involved in the regulation of NAD de novo biosynthesis in Salmonella typhimurium.

Journal of bacteriology ·Vol. 170 ·No. 1 ·1988-01-00 ·Pages 117-25

Zhu N, Olivera BM, Roth JR

Abstract

Mutations at the nadI locus affect expression of the first two genes of NAD synthesis, nadA and nadB, which are unlinked. Genetic data imply that the regulatory effects of nadI mutations are not due to indirect consequences of physiological alterations. Two types of mutations map in the nadI region. Common null mutations (nadI) show constitutive high-level expression of the nadB and nadA genes. Rare nadIs mutations cause constitutive low-level expression of nadB and nadA. Some nadIs mutations shut off the expression of the biosynthetic genes sufficiently to cause a nicotinic acid auxotrophy. Spontaneous revertants of auxotrophic nadIs mutants have a NadI- phenotype, including some with deletions of the nadI locus. The nadI locus encodes a repressor protein acting on the unlinked nadA and nadB genes.

MeSH Terms
Cloning, Molecular Gene Expression Regulation Genes, Bacterial Genes, Regulator Mutation NAD/biosynthesis,genetics Phenotype Repressor Proteins/genetics Salmonella typhimurium/genetics,metabolism Transcription Factors/genetics Transduction, Genetic
Chemicals
Repressor Proteins Transcription Factors NAD
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhu N
Department of Biology, University of Utah, Salt Lake City 84112.
Olivera B M
Roth J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.1.117-125.1988
Published
1988-01-00
Pages
117-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210614
Subset
IM
Grants
NIGMS NIH HHS · GM23408 · United States
NIGMS NIH HHS · GM25654 · United States
External Links
PubMed source DOI: 10.1128/jb.170.1.117-125.1988 PMC Full Text View journal details
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