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PMID: 3039308 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of NAD metabolism in Salmonella typhimurium: genetic analysis and cloning of the nadR repressor locus.

Molecular & general genetics : MGG ·Vol. 208 ·No. 1-2 ·1987-06-00 ·Pages 279-87

Foster JW, Holley-Guthrie EA, Warren F

Abstract

The nadR locus (99 min) controls the transcription of several genes involved with either the biosynthesis (nadAB) or recycling (pncB) of NAD in Salmonella typhimurium. Point mutations in this locus were found to cause defects either in the transport of nicotinamide mononucleotide (PnuA-), the regulation of nadAB (NadR-) or both transport and regulation (PnuA-NadR-). Deletions or insertions into nadR always resulted in the PnuA- NadR- phenotypes. Merodiploids constructed with various combinations of PnuA-, NadR- or PnuA- NadR- strains indicate a single complementation group. The results suggest the NadR product is a bifunctional regulatory protein. Operon fusions to lacZ (nadR :: Mud1-8) were used to show that nadR is not autoregulated and is transcribed in a clockwise direction. The gene was also cloned and located within a 2 kb EcoR1-Bg/II fragment.

MeSH Terms
Biological Transport Chromosome Deletion Cloning, Molecular DNA Transposable Elements Mutation NAD/metabolism Nicotinamide Mononucleotide/metabolism Operator Regions, Genetic Operon Oxygen/pharmacology Repressor Proteins/genetics Salmonella typhimurium/genetics,metabolism Transcription Factors/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements Repressor Proteins Transcription Factors NAD Nicotinamide Mononucleotide Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Foster J W
Holley-Guthrie E A
Warren F
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330454
Published
1987-06-00
Pages
279-87
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 32595 · United States
External Links
PubMed source DOI: 10.1007/BF00330454
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