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PMID: 3017917 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetics of NAD metabolism in Salmonella typhimurium and cloning of the nadA and pnuC loci.

Journal of bacteriology ·Vol. 167 ·No. 3 ·1986-09-00 ·Pages 1086-8

Tirgari S, Spector MP, Foster JW

Abstract

The nadA and pnuC loci of S. typhimurium were cloned and found to reside within a 2.2-kilobase region. Two-dimensional O'Farrell gel electrophoresis of the proteins produced after chloramphenicol amplification and subsequent release from chloramphenicol inhibition revealed NadA and PnuC to be 43,000- and 25,000-molecular-weight proteins, respectively. The data indicated that nadA and pnuC represent two distinct genes.

MeSH Terms
Bacterial Proteins/genetics,isolation & purification Genes, Bacterial Genetic Complementation Test NAD/metabolism Recombinant Proteins/genetics,isolation & purification Salmonella typhimurium/genetics,metabolism
Chemicals
Bacterial Proteins Recombinant Proteins NAD
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tirgari S
Spector M P
Foster J W
References (8)
8 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.3.1086-1088.1986
Published
1986-09-00
Pages
1086-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215988
Subset
IM
Grants
NIGMS NIH HHS · GM 32595 · United States
External Links
PubMed source DOI: 10.1128/jb.167.3.1086-1088.1986 PMC Full Text View journal details
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