Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 959-62

Holley EA, Foster JW

Abstract

Salmonella phage P22 was utilized as a vector for phage Mu cts d1(Apr lac) mutagenesis in Salmonella typhimurium. Efficient transposition of phage Mu d1 and the construction of gene fusions were readily accomplished with this procedure. Mutants blocked in the biosynthesis of NAD+ and in pyridine nucleotide cycle metabolism were isolated by this method, resulting in nadB-lac, nadC-lac, and pncB-lac gene fusions.

MeSH Terms

Genes, Bacterial Genes, Viral Genotype Mutation NAD/biosynthesis Phenotype Salmonella Phages/genetics Salmonella typhimurium/genetics Species Specificity

Chemicals

NAD

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Holley E A

Foster J W

References (17)

17 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.152.2.959-962.1982

Published

1982-11-00

Pages

959-62

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC221562

Subset

External Links

PubMed source DOI: 10.1128/jb.152.2.959-962.1982 PMC Full Text View journal details

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Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.

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