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PMID: 2834324 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural gene for NAD synthetase in Salmonella typhimurium.

Journal of bacteriology ·Vol. 170 ·No. 5 ·1988-05-00 ·Pages 2113-20

Hughes KT, Olivera BM, Roth JR

Abstract

We have identified the structural gene for NAD synthetase, which catalyzes the final metabolic step in NAD biosynthesis. This gene, designated nadE, is located between gdh and nit at 27 min on the Salmonella typhimurium chromosome. Mutants of nadE include those with a temperature-sensitive lethal phenotype; these strains accumulate large internal pools of nicotinic acid adenine dinucleotide, the substrate for NAD synthetase. Native gel electrophoresis experiments suggest that NAD synthetase is a multimeric enzyme of at least two subunits and that subunits from Escherichia coli and S. typhimurium interact to form an active heteromultimer.

MeSH Terms

Amide Synthases Chemical Phenomena Chemistry Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements Electrophoresis, Agar Gel Genes Lactose/metabolism Ligases/genetics,metabolism Mutation NAD/biosynthesis Pyrimidine Nucleotides/analysis Salmonella typhimurium/enzymology,genetics,growth & development Temperature Transduction, Genetic

Chemicals

DNA Transposable Elements Pyrimidine Nucleotides NAD Ligases Amide Synthases NAD+ synthase Lactose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hughes K T

Department of Biology, University of Utah, Salt Lake City 84112.

Olivera B M

Roth J R

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24 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.170.5.2113-2120.1988

Published

1988-05-00

Pages

2113-20

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC211094

Subset

Grants

NIGMS NIH HHS · GM23408 · United States

NIGMS NIH HHS · GM25654 · United States

NIGMS NIH HHS · T32-GM07537 · United States

External Links

PubMed source DOI: 10.1128/jb.170.5.2113-2120.1988 PMC Full Text View journal details

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Structural gene for NAD synthetase in Salmonella typhimurium.

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