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PMID: 2535735 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Deletions and insertions within an amino-terminal domain of pp60v-src inactivate transformation and modulate membrane stability.

Journal of virology ·Vol. 63 ·No. 1 ·1989-01-00 ·Pages 291-302

Wang HC, Parsons JT

Abstract

We previously showed (V. W. Raymond and J. T. Parsons, Virology 160:400-410, 1987) that variants of the Prague A strain of Rous sarcoma virus containing large deletions impinging on a region of the src gene encoding amino acid residues 143 to 169 were defective for transformation of chicken cells in culture. Here we report that introduction of small (tri-and tetrapeptide) deletions into a region of pp60v-src containing amino acid residues 155 to 175 was found to inactivate transformation. In addition, insertion of four, but not one, amino acid residues at position 161 also inhibited transformation. Biochemical analysis of the src proteins encoded by individual transformation-defective variants revealed that the structural alterations introduced into this domain had only marginal effects upon src tyrosine-specific protein kinase activity. However, the src proteins encoded by defective variants exhibited a significantly shorter half-life within the cell, although these proteins efficiently and rapidly associated with cellular membranes. Our results suggest that the structural domain encompassing residues 155 to 177 may influence the stability of pp60src in the cellular membrane, possibly via the interaction of src with a cellular membrane component(s) or substrate(s).

MeSH Terms
Amino Acid Sequence Animals Avian Sarcoma Viruses/genetics Cell Membrane/analysis,metabolism Cell Transformation, Viral Cells, Cultured Centrifugation, Density Gradient Molecular Sequence Data Mutation Oncogene Protein pp60(v-src) Precipitin Tests Protein Kinases/analysis Retroviridae Proteins/analysis,biosynthesis,genetics Transfection
Chemicals
Retroviridae Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang H C
Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908.
Parsons J T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.1.291-302.1989
Published
1989-01-00
Pages
291-302
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247684
Subset
IM
Grants
NCI NIH HHS · CA29243 · United States
NCI NIH HHS · CA40042 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.1.291-302.1989 PMC Full Text View journal details
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