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PMID: 24876254 Published · ppublish English Journal Article

Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.

The Plant cell ·Vol. 26 ·No. 5 ·2014-05-00 ·Pages 2114-2128

Tejos R, Sauer M, Vanneste S, Palacios-Gomez M, Li H, Heilmann M, van Wijk R, Vermeer JE, Heilmann I, Munnik T, Friml J

Abstract

Cell polarity manifested by asymmetric distribution of cargoes, such as receptors and transporters, within the plasma membrane (PM) is crucial for essential functions in multicellular organisms. In plants, cell polarity (re)establishment is intimately linked to patterning processes. Despite the importance of cell polarity, its underlying mechanisms are still largely unknown, including the definition and distinctiveness of the polar domains within the PM. Here, we show in Arabidopsis thaliana that the signaling membrane components, the phosphoinositides phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] as well as PtdIns4P 5-kinases mediating their interconversion, are specifically enriched at apical and basal polar plasma membrane domains. The PtdIns4P 5-kinases PIP5K1 and PIP5K2 are redundantly required for polar localization of specifically apical and basal cargoes, such as PIN-FORMED transporters for the plant hormone auxin. As a consequence of the polarity defects, instructive auxin gradients as well as embryonic and postembryonic patterning are severely compromised. Furthermore, auxin itself regulates PIP5K transcription and PtdIns4P and PtdIns(4,5)P2 levels, in particular their association with polar PM domains. Our results provide insight into the polar domain-delineating mechanisms in plant cells that depend on apical and basal distribution of membrane lipids and are essential for embryonic and postembryonic patterning.

Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Tejos Ricardo
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Sauer Michael
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Vanneste Steffen
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Palacios-Gomez Miriam
Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
Li Hongjiang
Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
Heilmann Mareike
Department of Cellular Biochemistry, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.
van Wijk Ringo
Swammerdam Institute for Life Sciences, Section Plant Physiology, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.
Vermeer Joop E M
Swammerdam Institute for Life Sciences, Section Plant Physiology, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.
Heilmann Ingo
Department of Cellular Biochemistry, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.
Munnik Teun
Swammerdam Institute for Life Sciences, Section Plant Physiology, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.
Friml Jiří
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria jiri.friml@ist.ac.at.
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.114.126185
Email
jiri.friml@ist.ac.at
Published
2014-05-00
Epub
2014-00-29
Pages
2114-2128
Language
English
Region
England
NLM ID
9208688
PMCID
PMC4079372
External Links
PubMed source DOI: 10.1105/tpc.114.126185 PMC Full Text View journal details
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