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PMID: 21551390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis.

The Plant cell ·Vol. 23 ·No. 5 ·2011-05-00 ·Pages 1920-31

Kitakura S, Vanneste S, Robert S, Löfke C, Teichmann T, Tanaka H, Friml J

Abstract

Endocytosis is a crucial mechanism by which eukaryotic cells internalize extracellular and plasma membrane material, and it is required for a multitude of cellular and developmental processes in unicellular and multicellular organisms. In animals and yeast, the best characterized pathway for endocytosis depends on the function of the vesicle coat protein clathrin. Clathrin-mediated endocytosis has recently been demonstrated also in plant cells, but its physiological and developmental roles remain unclear. Here, we assessed the roles of the clathrin-mediated mechanism of endocytosis in plants by genetic means. We interfered with clathrin heavy chain (CHC) function through mutants and dominant-negative approaches in Arabidopsis thaliana and established tools to manipulate clathrin function in a cell type-specific manner. The chc2 single mutants and dominant-negative CHC1 (HUB) transgenic lines were defective in bulk endocytosis as well as in internalization of prominent plasma membrane proteins. Interference with clathrin-mediated endocytosis led to defects in constitutive endocytic recycling of PIN auxin transporters and their polar distribution in embryos and roots. Consistent with this, these lines had altered auxin distribution patterns and associated auxin transport-related phenotypes, such as aberrant embryo patterning, imperfect cotyledon specification, agravitropic growth, and impaired lateral root organogenesis. Together, these data demonstrate a fundamental role for clathrin function in cell polarity, growth, patterning, and organogenesis in plants.

MeSH Terms
Arabidopsis/embryology,genetics,growth & development,physiology Arabidopsis Proteins/genetics,metabolism Biological Transport Cell Membrane/metabolism Cell Polarity Clathrin/metabolism Clathrin-Coated Vesicles/metabolism Endocytosis/physiology Genes, Reporter Indoleacetic Acids/metabolism Membrane Transport Proteins/metabolism Mutation Phenotype Plant Roots/genetics,growth & development,metabolism Plants, Genetically Modified/enzymology,genetics,growth & development,metabolism Recombinant Fusion Proteins Seedlings/genetics,growth & development,metabolism
Chemicals
Arabidopsis Proteins Clathrin Indoleacetic Acids Membrane Transport Proteins PIN1 protein, Arabidopsis PIN2 protein, Arabidopsis Recombinant Fusion Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kitakura Saeko
Department of Plant Systems Biology, VIB, Ghent, Belgium.
Vanneste Steffen
Robert Stéphanie
Löfke Christian
Teichmann Thomas
Tanaka Hirokazu
Friml Jirí
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.111.083030
Published
2011-05-00
Epub
2011-00-06
Pages
1920-31
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3123958
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.111.083030 PMC Full Text View journal details
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