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PMID: 21189293 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Phosphoinositides regulate clathrin-dependent endocytosis at the tip of pollen tubes in Arabidopsis and tobacco.

The Plant cell ·Vol. 22 ·No. 12 ·2010-12-00 ·Pages 4031-44

Zhao Y, Yan A, Feijó JA, Furutani M, Takenawa T, Hwang I, Fu Y, Yang Z

Abstract

Using the tip-growing pollen tube of Arabidopsis thaliana and Nicotiana tabacum as a model to investigate endocytosis mechanisms, we show that phosphatidylinositol-4-phosphate 5-kinase 6 (PIP5K6) regulates clathrin-dependent endocytosis in pollen tubes. Green fluorescent protein-tagged PIP5K6 was preferentially localized to the subapical plasma membrane (PM) in pollen tubes where it apparently converts phosphatidylinositol 4-phosphate (PI4P) to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P(2)]. RNA interference-induced suppression of PIP5K6 expression impaired tip growth and inhibited clathrin-dependent endocytosis in pollen tubes. By contrast, PIP5K6 overexpression induced massive aggregation of the PM in pollen tube tips. This PM abnormality was apparently due to excessive clathrin-dependent membrane invagination because this defect was suppressed by the expression of a dominant-negative mutant of clathrin heavy chain. These results support a role for PI(4,5)P(2) in promoting early stages of clathrin-dependent endocytosis (i.e., membrane invagination). Interestingly, the PIP5K6 overexpression-induced PM abnormality was partially suppressed not only by the overexpression of PLC2, which breaks down PI(4,5)P(2), but also by that of PI4Kβ1, which increases the pool of PI4P. Based on these observations, we propose that a proper balance between PI4P and PI(4,5)P(2) is required for clathrin-dependent endocytosis in the tip of pollen tubes.

MeSH Terms
Arabidopsis/physiology Arabidopsis Proteins/genetics Clathrin/physiology Endocytosis/physiology Phosphatidylinositols/physiology Pollen RNA Interference Tobacco/physiology
Chemicals
Arabidopsis Proteins Clathrin Phosphatidylinositols
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zhao Yan
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Yan An
Feijó José A
Furutani Masahiro
Takenawa Tadaomi
Hwang Inhwan
Fu Ying
Yang Zhenbiao
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.076760
Published
2010-12-00
Epub
2010-00-28
Pages
4031-44
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3027160
Subset
IM
Grants
NIGMS NIH HHS · R01 GM081451 · United States
NIGMS NIH HHS · GM081451 · United States
External Links
PubMed source DOI: 10.1105/tpc.110.076760 PMC Full Text View journal details
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