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PMID: 18591430 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth.

The Journal of cell biology ·Vol. 181 ·No. 7 ·2008-06-30 ·Pages 1155-68

Lee YJ, Szumlanski A, Nielsen E, Yang Z

Abstract

The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis.

MeSH Terms
Actin Cytoskeleton/metabolism Actins/metabolism Arabidopsis Proteins/metabolism Carrier Proteins Enzyme Activation Exocytosis Fluorescence Recovery After Photobleaching GTP-Binding Proteins/metabolism Models, Biological Pollen Tube/cytology,enzymology,growth & development Protein Transport Recombinant Fusion Proteins/metabolism Subcellular Fractions/metabolism Tobacco/cytology,enzymology,growth & development Transport Vesicles/metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Actins Arabidopsis Proteins Carrier Proteins RIC4 protein, Arabidopsis Recombinant Fusion Proteins GTP-Binding Proteins ROP1 protein, Arabidopsis rho GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee Yong Jik
Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA.
Szumlanski Amy
Nielsen Erik
Yang Zhenbiao
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200801086
Published
2008-06-30
Pages
1155-68
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2442199
Subset
IM
Grants
NIGMS NIH HHS · R01 GM081451 · United States
NIGMS NIH HHS · R01 GM081451-01 · United States
NIGMS NIH HHS · R01 GM081451-02 · United States
External Links
PubMed source DOI: 10.1083/jcb.200801086 PMC Full Text View journal details
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