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PMID: 11971147 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rab2 GTPase regulates vesicle trafficking between the endoplasmic reticulum and the Golgi bodies and is important to pollen tube growth.

The Plant cell ·Vol. 14 ·No. 4 ·2002-04-00 ·Pages 945-62

Cheung AY, Chen CY, Glaven RH, de Graaf BH, Vidali L, Hepler PK, Wu HM

Abstract

Pollen tube elongation depends on the secretion of large amounts of membrane and cell wall materials at the pollen tube tip to sustain rapid growth. A large family of RAS-related small GTPases, Rabs or Ypts, is known to regulate both anterograde and retrograde trafficking of transport vesicles between different endomembrane compartments and the plasma membrane in mammalian and yeast cells. Studies on the functional roles of analogous plant proteins are emerging. We report here that a tobacco pollen-predominant Rab2, NtRab2, functions in the secretory pathway between the endoplasmic reticulum and the Golgi in elongating pollen tubes. Green fluorescent protein-NtRab2 fusion protein localized to the Golgi bodies in elongating pollen tubes. Dominant-negative mutations in NtRab2 proteins inhibited their Golgi localization, blocked the delivery of Golgi-resident as well as plasmalemma and secreted proteins to their normal locations, and inhibited pollen tube growth. On the other hand, when green fluorescent protein-NtRab2 was over-expressed in transiently transformed leaf protoplasts and epidermal cells, in which NtRab2 mRNA have not been observed to accumulate to detectable levels, these proteins did not target efficiently to Golgi bodies. Together, these observations indicate that NtRab2 is important for trafficking between the endoplasmic reticulum and the Golgi bodies in pollen tubes and may be specialized to optimally support the high secretory demands in these tip growth cells.

MeSH Terms
Biological Transport Endoplasmic Reticulum/metabolism Gene Expression Regulation, Plant Golgi Apparatus/metabolism Green Fluorescent Proteins Luminescent Proteins/genetics,metabolism Microscopy, Electron Mutation Plant Leaves/genetics,metabolism Plants, Genetically Modified Pollen/genetics,growth & development,ultrastructure Recombinant Fusion Proteins/genetics,metabolism Tobacco/genetics,metabolism rab2 GTP-Binding Protein/genetics,physiology
Chemicals
Luminescent Proteins Recombinant Fusion Proteins Green Fluorescent Proteins rab2 GTP-Binding Protein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cheung Alice Y
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA. acheung@biochem.umass.edu
Chen Christine Y-h
Glaven Richard H
de Graaf Barend H J
Vidali Luis
Hepler Peter K
Wu Hen-ming
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.000836
Email
acheung@biochem.umass.edu
Published
2002-04-00
Pages
945-62
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150694
Subset
IM
Grants
CGH CDC HHS · GH52953 · United States
External Links
PubMed source DOI: 10.1105/tpc.000836 PMC Full Text View journal details
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