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PMID: 10209027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth.

The Journal of cell biology ·Vol. 145 ·No. 2 ·1999-04-19 ·Pages 317-30

Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH

Abstract

Pollen tube cells elongate based on actin- dependent targeted secretion at the tip. Rho family small GTPases have been implicated in the regulation of related processes in animal and yeast cells. We have functionally characterized Rac type Rho family proteins that are expressed in growing pollen tubes. Expression of dominant negative Rac inhibited pollen tube elongation, whereas expression of constitutive active Rac induced depolarized growth. Pollen tube Rac was found to accumulate at the tip plasma membrane and to physically associate with a phosphatidylinositol monophosphate kinase (PtdIns P-K) activity. Phosphatidylinositol 4, 5-bisphosphate (PtdIns 4, 5-P2), the product of PtdIns P-Ks, showed a similar intracellular localization as Rac. Expression of the pleckstrin homology (PH)-domain of phospholipase C (PLC)-delta1, which binds specifically to PtdIns 4, 5-P2, inhibited pollen tube elongation. These results indicate that Rac and PtdIns 4, 5-P2 act in a common pathway to control polar pollen tube growth and provide direct evidence for a function of PtdIns 4, 5-P2 compartmentalization in the regulation of this process.

MeSH Terms
Amino Acid Sequence Arabidopsis/physiology,ultrastructure Cells, Cultured GTP Phosphohydrolases/metabolism GTP-Binding Proteins/chemistry,genetics,metabolism Glycosylation Isoenzymes/metabolism Molecular Sequence Data Phosphatidylinositol 4,5-Diphosphate/metabolism Phospholipase C delta Plant Proteins/metabolism Plant Structures Plants, Toxic Pollen/physiology,ultrastructure Recombinant Proteins/metabolism Sequence Alignment Sequence Homology, Amino Acid Tobacco Transfection Type C Phospholipases/metabolism rac GTP-Binding Proteins
Chemicals
Isoenzymes Phosphatidylinositol 4,5-Diphosphate Plant Proteins Recombinant Proteins Type C Phospholipases Phospholipase C delta GTP Phosphohydrolases GTP-Binding Proteins rac GTP-Binding Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kost B
Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399, USA.
Lemichez E
Spielhofer P
Hong Y
Tolias K
Carpenter C
Chua N H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.145.2.317
Published
1999-04-19
Pages
317-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133117
Subset
IM
Grants
NIGMS NIH HHS · R01 GM054389 · United States
NIGMS NIH HHS · GM54389 · United States
External Links
PubMed source DOI: 10.1083/jcb.145.2.317 PMC Full Text View journal details
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