Home LiteratureArticle Details
PMID: 9765526 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast.

Plant physiology ·Vol. 118 ·No. 2 ·1998-10-00 ·Pages 407-17

Li H, Wu G, Ware D, Davis KR, Yang Z

Abstract

The Rho small GTP-binding proteins are versatile, conserved molecular switches in eukaryotic signal transduction. Plants contain a unique subfamily of Rho-GTPases called Rop (Rho-related GTPases from plants). Our previous studies involving injection of antibodies indicated that the pea Rop GTPase Rop1Ps is critical for pollen tube growth. In this study we show that overexpression of an apparent Arabidopsis ortholog of Rop1Ps, Rop1At, induces isotropic cell growth in fission yeast (Schizosaccharomyces pombe) and that green fluorescence protein-tagged Rop1At displays polar localization to the site of growth in yeast. We found that Rop1At and two other Arabidopsis Rops, Rop3At and Rop5At, are all expressed in mature pollen. All three pollen Rops fall into the same subgroup as Rop1Ps and diverge from those Rops that are not expressed in mature pollen, suggesting a coupling of the structural conservation of Rop GTPases to their gene expression in pollen. However, pollen-specific transcript accumulation for Rop1At is much higher than that for Rop3At and Rop5At. Furthermore, Rop1At is specifically expressed in anthers, whereas Rop3At and Rop5At are also expressed in vegetative tissues. In transgenic plants containing the Rop1At promoter:GUS fusion gene, GUS is specifically expressed in mature pollen and pollen tubes. We propose that Rop1At may play a predominant role in the regulation of polarized cell growth in pollen, whereas its close relatives Rop3At and Rop5At may be functionally redundant to Rop1At in pollen.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Base Sequence Cell Division/genetics Cloning, Molecular GTP Phosphohydrolases/genetics,metabolism GTP-Binding Proteins/metabolism Gene Expression Regulation, Fungal Gene Expression Regulation, Plant Molecular Sequence Data Pollen/enzymology Schizosaccharomyces/genetics Sequence Homology, Amino Acid
Chemicals
GTP Phosphohydrolases GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li H
Department of Plant Biology and Plant Biotechnology Center, The Ohio State University, Columbus, Ohio 43210, USA.
Wu G
Ware D
Davis K R
Yang Z
References (50)
50 references, click to expand
  1. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors.
    Cell. 1992 Aug 7;70(3):389-99 PMID: 1643657
  2. High efficiency transformation of Schizosaccharomyces pombe by electroporation.
    Nucleic Acids Res. 1992 Feb 11;20(3):621 PMID: 1741305
  3. Targeted disruption in Arabidopsis.
    Nature. 1997 Oct 23;389(6653):802-3 PMID: 9349810
  4. Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion.
    Genes Dev. 1994 Aug 1;8(15):1787-802 PMID: 7958857
  5. Sequence-based identification of T-DNA insertion mutations in Arabidopsis: actin mutants act2-1 and act4-1.
    Plant J. 1995 Oct;8(4):613-22 PMID: 7496405
  6. Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.
    Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2122-7 PMID: 9122158
  7. Improved method for the isolation of RNA from plant tissues.
    Anal Biochem. 1987 May 15;163(1):16-20 PMID: 2441623
  8. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling.
    Cell. 1992 Aug 7;70(3):401-10 PMID: 1643658
  9. Cloning and characterization of rac-like cDNAs from Arabidopsis thaliana.
    Plant Mol Biol. 1997 Nov;35(4):483-95 PMID: 9349271
  10. Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase.
    Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8837-41 PMID: 8090732
  11. POLLEN GERMINATION AND TUBE GROWTH.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:461-491 PMID: 15012271
  12. Endosome dynamics regulated by a Rho protein.
    Nature. 1996 Dec 5;384(6608):427-32 PMID: 8945468
  13. Regulation of receptor-mediated endocytosis by Rho and Rac.
    Nature. 1996 Jul 11;382(6587):177-9 PMID: 8700210
  14. Rho: theme and variations.
    Curr Biol. 1996 Oct 1;6(10):1256-64 PMID: 8939567
  15. Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8145-50 PMID: 8755618
  16. The Arabidopsis thaliana ACT4/ACT12 actin gene subclass is strongly expressed throughout pollen development.
    Plant J. 1996 Aug;10(2):189-202 PMID: 8771777
  17. A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.
    J Cell Biol. 1996 Jun;133(6):1321-9 PMID: 8682867
  18. Intracellular localization of the P21rho proteins.
    J Cell Biol. 1992 Nov;119(3):617-27 PMID: 1383236
  19. Early flower development in Arabidopsis.
    Plant Cell. 1990 Aug;2(8):755-67 PMID: 2152125
  20. Genes encoding small GTP-binding proteins analogous to mammalian rac are preferentially expressed in developing cotton fibers.
    Mol Gen Genet. 1995 Jul 22;248(1):43-51 PMID: 7651326
  21. Pollen- and anther-specific chi promoters from petunia: tandem promoter regulation of the chiA gene.
    Plant Cell. 1990 May;2(5):393-401 PMID: 2152165
  22. Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements.
    Genes Dev. 1991 Mar;5(3):496-507 PMID: 1840556
  23. Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe.
    Mol Cell Biol. 1994 Feb;14(2):1075-83 PMID: 8289788
  24. Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth.
    Plant Cell. 1997 Sep;9(9):1647-1659 PMID: 12237397
  25. Molecular Mechanisms of Pollen Tube Growth and Differentiation.
    Plant Cell. 1993 Oct;5(10):1303-1314 PMID: 12271030
  26. Identification of plant cytoskeletal, cell cycle-related and polarity-related proteins using Schizosaccharomyces pombe.
    Plant J. 1996 Oct;10(4):761-9 PMID: 8893552
  27. A developmentally regulated MAP kinase activated by hydration in tobacco pollen.
    Plant Cell. 1997 Nov;9(11):2093-100 PMID: 9401129
  28. Promoter sequences of two homologous pectin esterase genes from Chinese cabbage (Brassica campestris L. ssp. pekinensis) and pollen-specific expression of the GUS gene driven by a promoter in tobacco plants.
    Mol Cells. 1997 Feb 28;7(1):21-7 PMID: 9085260
  29. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  30. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  31. Phytochrome control of RNA levels in developing pea and mung-bean leaves.
    Planta. 1983 Aug;158(6):487-500 PMID: 24264922
  32. Arabidopsis profilins are functionally similar to yeast profilins: identification of a vascular bundle-specific profilin and a pollen-specific profilin.
    Plant J. 1996 Aug;10(2):269-79 PMID: 8771785
  33. The structure, distribution and evolution of the Ta1 retrotransposable element family of Arabidopsis thaliana.
    Genetics. 1990 Nov;126(3):713-21 PMID: 2174394
  34. Comparison of Schizosaccharomyces pombe expression systems.
    Nucleic Acids Res. 1993 Jun 25;21(12):2955-6 PMID: 8332516
  35. Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues.
    Plant J. 1996 Jul;10(1):107-21 PMID: 8758981
  36. The Arabidopsis profilin gene family. Evidence for an ancient split between constitutive and pollen-specific profilin genes.
    Plant Physiol. 1996 May;111(1):115-26 PMID: 8685262
  37. The Arabidopsis ACT11 actin gene is strongly expressed in tissues of the emerging inflorescence, pollen, and developing ovules.
    Plant Mol Biol. 1997 Jan;33(1):125-39 PMID: 9037165
  38. Structure and evolution of the actin gene family in Arabidopsis thaliana.
    Genetics. 1996 Feb;142(2):587-602 PMID: 8852856
  39. GTPase cascades choreographing cellular behavior: movement, morphogenesis, and more.
    Cell. 1995 Apr 7;81(1):1-4 PMID: 7720065
  40. Molecular cloning and characterization of rho, a ras-related small GTP-binding protein from the garden pea.
    Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8732-6 PMID: 8378356
  41. Conserved expression of the Arabidopsis ACT1 and ACT 3 actin subclass in organ primordia and mature pollen.
    Plant Cell. 1996 Jan;8(1):15-30 PMID: 8597657
  42. A CAAX or a CAAL motif and a second signal are sufficient for plasma membrane targeting of ras proteins.
    EMBO J. 1991 Dec;10(13):4033-9 PMID: 1756714
  43. The profilin multigene family of maize: differential expression of three isoforms.
    Plant J. 1993 Oct;4(4):631-41 PMID: 8252067
  44. Localization of a Rho GTPase Implies a Role in Tip Growth and Movement of the Generative Cell in Pollen Tubes.
    Plant Cell. 1996 Feb;8(2):293-303 PMID: 12239385
  45. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536-40 PMID: 16593964
  46. Male Gametophyte Development.
    Plant Cell. 1993 Oct;5(10):1265-1275 PMID: 12271026
  47. Small GTP-binding proteins and the regulation of the actin cytoskeleton.
    Annu Rev Cell Biol. 1994;10:31-54 PMID: 7888179
  48. Rac binding to p67(phox). Structural basis for interactions of the Rac1 effector region and insert region with components of the respiratory burst oxidase.
    J Biol Chem. 1997 Jul 25;272(30):18834-41 PMID: 9228059
  49. Applications of fluorochromes to pollen biology. I. Mithramycin and 4',6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA.
    Stain Technol. 1985 May;60(3):145-54 PMID: 2411019
  50. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.
    Cell. 1995 Apr 7;81(1):53-62 PMID: 7536630
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.118.2.407
Published
1998-10-00
Pages
407-17
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC34816
Subset
IM
Grants
NIGMS NIH HHS · GM 45570 · United States
Databases
GENBANK
AF031427, AF031428, AF064082, U49971, U49972
External Links
PubMed source DOI: 10.1104/pp.118.2.407 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2