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PMID: 11387211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth.

The EMBO journal ·Vol. 20 ·No. 11 ·2001-06-01 ·Pages 2779-88

Molendijk AJ, Bischoff F, Rajendrakumar CS, Friml J, Braun M, Gilroy S, Palme K

Abstract

Plants contain a novel unique subfamily of Rho GTPases, vital components of cellular signalling networks. Here we report a general role for some members of this family in polarized plant growth processes. We show that Arabidopsis AtRop4 and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates. We localized AtRop proteins in root meristem cells to the cross-wall and cell plate membranes. Polar localization of AtRops in trichoblasts specifies the growth sites for emerging root hairs. These sites were visible before budding and elongation of the Arabidopsis root hair when AtRops accumulated at their tips. Expression of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic Arabidopsis plants abolished polarized growth and delocalized the tip-focused Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse growth.

Keywords
Non-programmatic
MeSH Terms
Arabidopsis/cytology,enzymology,growth & development Calcium/metabolism Cell Line Cytoskeleton/enzymology,ultrastructure GTP Phosphohydrolases/genetics,metabolism Immunohistochemistry Microtubules/enzymology,ultrastructure Plant Proteins/metabolism Plant Roots/cytology,enzymology,growth & development Plants, Toxic Recombinant Proteins/metabolism Tobacco Transfection
Chemicals
Plant Proteins Recombinant Proteins GTP Phosphohydrolases Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Molendijk A J
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft and Max-Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany.
Bischoff F
Rajendrakumar C S
Friml J
Braun M
Gilroy S
Palme K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.11.2779
Published
2001-06-01
Pages
2779-88
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125484
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.11.2779 PMC Full Text View journal details
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