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PMID: 10072399 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein.

The Plant cell ·Vol. 11 ·No. 3 ·1999-03-00 ·Pages 393-406

Trotochaud AE, Hao T, Wu G, Yang Z, Clark SE

Abstract

The CLAVATA1 (CLV1) and CLAVATA3 (CLV3) genes are required to maintain the balance between cell proliferation and organ formation at the Arabidopsis shoot and flower meristems. CLV1 encodes a receptor-like protein kinase. We have found that CLV1 is present in two protein complexes in vivo. One is approximately 185 kD, and the other is approximately 450 kD. In each complex, CLV1 is part of a disulfide-linked multimer of approximately 185 kD. The 450-kD complex contains the protein phosphatase KAPP, which is a negative regulator of CLV1 signaling, and a Rho GTPase-related protein. In clv1 and clv3 mutants, CLV1 is found primarily in the 185-kD complex. We propose that CLV1 is present as an inactive disulfide-linked heterodimer and that CLV3 functions to promote the assembly of the active 450-kD complex, which then relays signal transduction through a Rho GTPase.

MeSH Terms
Arabidopsis Proteins Brassica/genetics,metabolism Disulfides/metabolism Enzyme-Linked Immunosorbent Assay GTP-Binding Proteins/metabolism GTPase-Activating Proteins Phosphoprotein Phosphatases/genetics,metabolism Plant Proteins/genetics,metabolism Protein Binding Protein Serine-Threonine Kinases RNA, Messenger/metabolism Receptor Protein-Tyrosine Kinases/genetics,metabolism Signal Transduction
Chemicals
AT2G27250 protein, Arabidopsis Arabidopsis Proteins Disulfides GTPase-Activating Proteins Plant Proteins RNA, Messenger rho GTPase-activating protein Receptor Protein-Tyrosine Kinases CLV1 protein, Arabidopsis Protein Serine-Threonine Kinases KAPP protein, Arabidopsis Phosphoprotein Phosphatases GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Trotochaud A E
Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA.
Hao T
Wu G
Yang Z
Clark S E
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.11.3.393
Published
1999-03-00
Pages
393-406
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144183
Subset
IM
Corrections
CommentIn
PMID: 10072390
External Links
PubMed source DOI: 10.1105/tpc.11.3.393 PMC Full Text View journal details
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