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PMID: 11971133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The Arabidopsis Rop2 GTPase is a positive regulator of both root hair initiation and tip growth.

The Plant cell ·Vol. 14 ·No. 4 ·2002-04-00 ·Pages 763-76

Jones MA, Shen JJ, Fu Y, Li H, Yang Z, Grierson CS

Abstract

Root hairs provide a model system for the study of cell polarity. We examined the possibility that one or more members of the distinct plant subfamily of RHO monomeric GTPases, termed Rop, may function as molecular switches regulating root hair growth. Specific Rops are known to control polar growth in pollen tubes. Overexpressing Rop2 (Rop2 OX) resulted in a strong root hair phenotype, whereas overexpressing Rop7 appeared to inhibit root hair tip growth. Overexpressing Rops from other phylogenetic subgroups of Rop did not give a root hair phenotype. We confirmed that Rop2 was expressed throughout hair development. Rop2 OX and constitutively active GTP-bound rop2 (CA-rop2) led to additional and misplaced hairs on the cell surface as well as longer hairs. Furthermore, CA-rop2 depolarized root hair tip growth, whereas Rop2 OX resulted in hairs with multiple tips. Dominant negative GDP-bound Rop2 reduced the number of hair-forming sites and led to shorter and wavy hairs. Green fluorescent protein-Rop2 localized to the future site of hair formation well before swelling formation and to the tip throughout hair development. We conclude that the Arabidopsis Rop2 GTPase acts as a positive regulatory switch in the earliest visible stage in hair development, swelling formation, and in tip growth.

MeSH Terms
Actins/metabolism Arabidopsis/enzymology,genetics,growth & development Arabidopsis Proteins/genetics,metabolism Cell Differentiation Cytoskeleton/metabolism GTPase-Activating Proteins/genetics,metabolism Gene Expression Green Fluorescent Proteins Luminescent Proteins/drug effects,metabolism Phenotype Plant Epidermis/cytology,enzymology,genetics Plant Structures/cytology,enzymology,genetics Plants, Genetically Modified Recombinant Fusion Proteins/genetics,metabolism Signal Transduction rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Actins Arabidopsis Proteins GTPase-Activating Proteins Luminescent Proteins Recombinant Fusion Proteins Green Fluorescent Proteins rho GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jones Mark A
School of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom.
Shen Jun-Jiang
Fu Ying
Li Hai
Yang Zhenbiao
Grierson Claire S
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010359
Published
2002-04-00
Pages
763-76
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150680
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.010359 PMC Full Text View journal details
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