Home LiteratureArticle Details
PMID: 2164028 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.

The Journal of cell biology ·Vol. 111 ·No. 1 ·1990-07-00 ·Pages 143-52

Johnson DI, Pringle JR

Abstract

The Saccharomyces cerevisiae CDC42 gene product is involved in the morphogenetic events of the cell division cycle; temperature-sensitive cdc42 mutants are unable to form buds and display delocalized cell-surface deposition at the restrictive temperature (Adams, A. E. M., D. I. Johnson, R. M. Longnecker, B. F. Sloat, and J. R. Pringle. 1990. J. Cell Biol. 111:131-142). To begin a molecular analysis of CDC42 function, we have isolated the CDC42 gene from a yeast genomic DNA library. The use of the cloned DNA to create a deletion of CDC42 confirmed that the gene is essential. Overexpression of CDC42 under control of the GAL10 promoter was not grossly deleterious to cell growth but did perturb the normal pattern of selection of budding sites. Determination of the DNA and predicted amino acid sequences of CDC42 revealed a high degree of similarity in amino acid sequence to the ras and rho (Madaule, P., R. Axel, and A. M. Myers. 1987. Proc. Natl. Acad. Sci. 84:779-783) families of gene products. The similarities to ras proteins (approximately 40% identical or related amino acids overall) were most pronounced in the regions that have been implicated in GTP binding and hydrolysis and in the COOH-terminal modifications leading to membrane association, suggesting that CDC42 function also involves these biochemical properties. The similarities to the rho proteins (approximately 60% identical or related amino acids overall) were more widely distributed through the coding region, suggesting more extensive similarities in as yet undefined biochemical properties and functions.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular DNA Transposable Elements DNA, Fungal/genetics,isolation & purification Fungal Proteins/genetics Gene Library Genes, Fungal Molecular Sequence Data Morphogenesis Nucleic Acid Hybridization RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/cytology,genetics,physiology Sequence Homology, Nucleic Acid Transcription, Genetic cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Chemicals
DNA Transposable Elements DNA, Fungal Fungal Proteins RNA, Messenger cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johnson D I
Department of Biology, University of Michigan, Ann Arbor 48109.
Pringle J R
References (58)
58 references, click to expand
  1. The ras-like yeast YPT1 gene is itself essential for growth, sporulation, and starvation response.
    Mol Cell Biol. 1987 Jul;7(7):2367-77 PMID: 3302675
  2. Nucleotide sequence of the CLS4 (CDC24) gene of Saccharomyces cerevisiae.
    Gene. 1987;54(1):125-32 PMID: 3301539
  3. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction.
    Cell. 1987 Sep 25;50(7):1011-9 PMID: 3113739
  4. Biochemical properties of the ras-related YPT protein in yeast: a mutational analysis.
    EMBO J. 1987 Aug;6(8):2373-9 PMID: 3311726
  5. Expression of the Aplysia californica rho gene in Escherichia coli: purification and characterization of its encoded p21 product.
    Mol Cell Biol. 1987 Oct;7(10):3620-8 PMID: 3119990
  6. Identification and purification from bovine brain of a guanine-nucleotide-binding protein distinct from Gs, Gi and Go.
    Biochem J. 1987 Sep 1;246(2):431-9 PMID: 3120696
  7. Three-dimensional structure of an oncogene protein: catalytic domain of human c-H-ras p21.
    Science. 1988 Feb 19;239(4842):888-93 PMID: 2448879
  8. Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1374-8 PMID: 2830616
  9. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.
    Cell. 1988 Mar 25;52(6):915-24 PMID: 3127057
  10. A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast.
    Cell. 1988 Jun 3;53(5):753-68 PMID: 3131018
  11. Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4620-4 PMID: 3133654
  12. Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4643-7 PMID: 3290900
  13. A yeast gene required for the G1-to-S transition encodes a protein containing an A-kinase target site and GTPase domain.
    EMBO J. 1988 Apr;7(4):1175-82 PMID: 2841115
  14. A carboxyl-terminal cysteine residue is required for palmitic acid binding and biological activity of the ras-related yeast YPT1 protein.
    EMBO J. 1988 Apr;7(4):971-6 PMID: 3042385
  15. Calcium-sensitive cls4 mutant of Saccharomyces cerevisiae with a defect in bud formation.
    J Bacteriol. 1986 Jan;165(1):28-33 PMID: 3510189
  16. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9 PMID: 3003748
  17. Structure of yeast LEU4. The 5' flanking region contains features that predict two modes of control and two productive translation starts.
    J Biol Chem. 1986 Apr 15;261(11):5160-7 PMID: 2420798
  18. RAS genes and growth control in Saccharomyces cerevisiae.
    J Bacteriol. 1986 May;166(2):364-7 PMID: 3516972
  19. Purification of the major GTP-binding proteins from human placental membranes.
    J Biol Chem. 1986 May 25;261(15):7052-9 PMID: 3084494
  20. Isolation and characterization of Ca2+-sensitive mutants of Saccharomyces cerevisiae.
    J Gen Microbiol. 1986 Apr;132(4):979-88 PMID: 3531397
  21. Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 May;6(5):1590-8 PMID: 3537706
  22. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome.
    Mol Cell Biol. 1986 Dec;6(12):4516-25 PMID: 3540615
  23. Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):779-83 PMID: 3543936
  24. GTP-binding domain: three consensus sequence elements with distinct spacing.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1814-8 PMID: 3104905
  25. Mapping of the Saccharomyces cerevisiae CDC3, CDC25, and CDC42 genes to chromosome XII by chromosome blotting and tetrad analysis.
    Yeast. 1987 Dec;3(4):243-53 PMID: 3332976
  26. Yeast actin-binding proteins: evidence for a role in morphogenesis.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2551-61 PMID: 3060468
  27. Structure and expression of yeast DPR1, a gene essential for the processing and intracellular localization of ras proteins.
    Yeast. 1988 Dec;4(4):271-81 PMID: 3064491
  28. Characterization of G25K, a GTP-binding protein containing a novel putative nucleotide binding domain.
    Biochem Biophys Res Commun. 1989 Apr 14;160(1):25-32 PMID: 2496687
  29. All ras proteins are polyisoprenylated but only some are palmitoylated.
    Cell. 1989 Jun 30;57(7):1167-77 PMID: 2661017
  30. Genetic and pharmacological suppression of oncogenic mutations in ras genes of yeast and humans.
    Science. 1989 Jul 28;245(4916):379-85 PMID: 2569235
  31. rac, a novel ras-related family of proteins that are botulinum toxin substrates.
    J Biol Chem. 1989 Oct 5;264(28):16378-82 PMID: 2674130
  32. Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9976-80 PMID: 2690082
  33. CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae.
    J Cell Biol. 1990 Jul;111(1):131-42 PMID: 2195038
  34. Genetic control of the cell division cycle in yeast.
    Science. 1974 Jan 11;183(4120):46-51 PMID: 4587263
  35. Transient G1 arrest of S. cerevisiae cells of mating type alpha by a factor produced by cells of mating type a.
    Exp Cell Res. 1974 Nov;89(1):175-87 PMID: 4611778
  36. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  37. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  38. A mutant of yeast defective in cellular morphogenesis.
    Science. 1978 Jun 9;200(4346):1171-3 PMID: 349694
  39. Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):343-7 PMID: 154672
  40. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  41. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
    Gene. 1979 Dec;8(1):17-24 PMID: 395030
  42. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation.
    J Bacteriol. 1980 Sep;143(3):1384-94 PMID: 6997270
  43. Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae.
    J Cell Biol. 1980 Jul;86(1):123-8 PMID: 6998984
  44. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  45. Roles of the CDC24 gene product in cellular morphogenesis during the Saccharomyces cerevisiae cell cycle.
    J Cell Biol. 1981 Jun;89(3):395-405 PMID: 7019215
  46. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  47. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  48. Yeast centromere DNA is in a unique and highly ordered structure in chromosomes and small circular minichromosomes.
    Cell. 1982 Jun;29(2):305-17 PMID: 6288253
  49. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  50. A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.
    Nature. 1983 Dec 15-21;306(5944):704-7 PMID: 6318115
  51. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
    Cell. 1984 Mar;36(3):607-12 PMID: 6365329
  52. Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.
    Cell. 1984 Mar;36(3):645-53 PMID: 6365330
  53. Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.
    J Cell Biol. 1984 Mar;98(3):934-45 PMID: 6365931
  54. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  55. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  56. A novel ras-related gene family.
    Cell. 1985 May;41(1):31-40 PMID: 3888408
  57. A ras-like protein is required for a post-Golgi event in yeast secretion.
    Cell. 1987 May 22;49(4):527-38 PMID: 3552249
  58. The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway.
    Cell. 1987 Sep 25;50(7):1001-10 PMID: 3113738
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.1.143
Published
1990-07-00
Pages
143-52
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116164
Subset
IM
Grants
NIGMS NIH HHS · GM-31006 · United States
Databases
GENBANK
X51906
External Links
PubMed source DOI: 10.1083/jcb.111.1.143 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2