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PMID: 2476805 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 6925-9

Dingwall C, Ernberg I, Gait MJ, Green SM, Heaphy S, Karn J, Lowe AD, Singh M, Skinner MA, Valerio R

Abstract

tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.

MeSH Terms
Base Sequence Escherichia coli/genetics Gene Products, tat Genes Genes, Synthetic Genes, Viral HIV-1/genetics,metabolism HeLa Cells/metabolism Humans Kinetics Molecular Sequence Data Nucleic Acid Conformation Oncogene Proteins, Viral/metabolism Plasmids Protein Binding RNA/metabolism RNA, Antisense Recombinant Proteins/isolation & purification,metabolism Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Oncogene Proteins, Viral RNA, Antisense Recombinant Proteins Transcription Factors tat Gene Products, Human Immunodeficiency Virus RNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Dingwall C
Medical Research Council Laboratory of Molecular Biology, Cambridge, England.
Ernberg I
Gait M J
Green S M
Heaphy S
Karn J
Lowe A D
Singh M
Skinner M A
Valerio R
References (31)
31 references, click to expand
  1. Tat protein from human immunodeficiency virus forms a metal-linked dimer.
    Science. 1988 Apr 1;240(4848):70-3 PMID: 2832944
  2. A high-efficiency HeLa cell nuclear transcription extract.
    DNA. 1988 Jan-Feb;7(1):47-55 PMID: 3349904
  3. Mutational analysis of the 5' non-coding region of human immunodeficiency virus type 1: effects of secondary structure on translation.
    EMBO J. 1988 Sep;7(9):2831-7 PMID: 3181141
  4. Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.
    Genes Dev. 1988 Sep;2(9):1101-14 PMID: 2847959
  5. Autonomous functional domains of chemically synthesized human immunodeficiency virus tat trans-activator protein.
    Cell. 1988 Dec 23;55(6):1179-88 PMID: 2849509
  6. Cellular uptake of the tat protein from human immunodeficiency virus.
    Cell. 1988 Dec 23;55(6):1189-93 PMID: 2849510
  7. Activation of double-stranded RNA-dependent kinase (dsl) by the TAR region of HIV-1 mRNA: a novel translational control mechanism.
    Cell. 1989 Jan 27;56(2):303-12 PMID: 2536299
  8. Developmental expression of a neurofilament-M and two vimentin-like genes in Xenopus laevis.
    Development. 1988 Jun;103(2):269-77 PMID: 3224553
  9. Cloning and expression in E. coli of a synthetic gene for the bacteriocidal protein caltrin/seminalplasmin.
    Protein Eng. 1987 Oct-Nov;1(5):425-31 PMID: 3334097
  10. Human immunodeficiency virus type 1 LTR TATA and TAR region sequences required for transcriptional regulation.
    EMBO J. 1989 Mar;8(3):765-78 PMID: 2721501
  11. Regulation of cell cycle duration by c-myc levels.
    Oncogene. 1989 Jun;4(6):773-87 PMID: 2660073
  12. Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
    Cell. 1982 Dec;31(2 Pt 1):395-405 PMID: 6297765
  13. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  14. Sequence-specific interaction of R17 coat protein with its ribonucleic acid binding site.
    Biochemistry. 1983 May 24;22(11):2601-10 PMID: 6347247
  15. Easy identification of cDNA clones.
    EMBO J. 1983;2(10):1791-4 PMID: 6315402
  16. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  17. A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction.
    Science. 1985 Jan 4;227(4682):23-8 PMID: 2981116
  18. Nucleotide sequence of the AIDS virus, LAV.
    Cell. 1985 Jan;40(1):9-17 PMID: 2981635
  19. Trans-activator gene of human T-lymphotropic virus type III (HTLV-III).
    Science. 1985 Jul 5;229(4708):69-73 PMID: 2990040
  20. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  21. The trans-activator gene of the human T cell lymphotropic virus type III is required for replication.
    Cell. 1986 Mar 28;44(6):941-7 PMID: 2420471
  22. Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors.
    Cell. 1986 Aug 15;46(4):567-74 PMID: 3524858
  23. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  24. Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein.
    Cell. 1987 Feb 27;48(4):691-701 PMID: 3643816
  25. Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
    EMBO J. 1987 Jan;6(1):69-74 PMID: 2884102
  26. Trans-activation of human immunodeficiency virus gene expression is mediated by nuclear events.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6364-8 PMID: 3476953
  27. Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
    Nature. 1987 Dec 3-9;330(6147):489-93 PMID: 2825027
  28. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
    Nucleic Acids Res. 1987 Nov 11;15(21):8783-98 PMID: 3684574
  29. Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.
    EMBO J. 1987 Dec 1;6(12):3761-70 PMID: 3428273
  30. Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli.
    Methods Enzymol. 1987;153:461-81 PMID: 3323806
  31. HIV-1 tat trans-activation requires the loop sequence within tar.
    Nature. 1988 Jul 14;334(6178):165-7 PMID: 3386755
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.6925
Published
1989-09-00
Pages
6925-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297963
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.18.6925 PMC Full Text View journal details
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