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PMID: 3428273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.

The EMBO journal ·Vol. 6 ·No. 12 ·1987-12-01 ·Pages 3761-70

Garcia JA, Wu FK, Mitsuyasu R, Gaynor RB

Abstract

The human immunodeficiency virus (HIV) is a human retrovirus which is the etiologic agent of the acquired immunodeficiency syndrome. To study the cellular factors involved in the transcriptional regulation of this virus, we performed DNase I footprinting of the viral LTR using partially purified HeLa cell extracts. Five regions of the viral LTR appear critical for DNA binding of cellular proteins. These include the negative regulatory, enhancer, SP1, TATA and untranslated regions. Deletion mutagenesis of these binding domains has significant effects on the basal level of transcription and the ability to be induced by the viral tat protein. Mutations of either the negative regulatory or untranslated regions affect factor binding to the enhancer region. In addition, oligonucleotides complementary to several of the binding domains specifically compete for factor binding. These results suggest that interactions between several distinct cellular proteins are required for HIV transcriptional regulation.

MeSH Terms
Chromosome Deletion Gene Expression Regulation Genes Genes, Viral HIV/genetics HeLa Cells/metabolism Humans Mutation Neoplasm Proteins/physiology Transcription Factors/genetics Transcription, Genetic Transfection
Chemicals
Neoplasm Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garcia J A
Department of Medicine, UCLA School of Medicine 90024.
Wu F K
Mitsuyasu R
Gaynor R B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-01
Pages
3761-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553847
Subset
IM
Grants
NCI NIH HHS · CA32737 · United States
NCI NIH HHS · CA3981 · United States
NIGMS NIH HHS · GMO-80942 · United States
External Links
PubMed source PMC Full Text View journal details
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