Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9759-63

Gendelman HE, Phelps W, Feigenbaum L, Ostrove JM, Adachi A, Howley PM, Khoury G, Ginsberg HS, Martin MA

Abstract

To investigate whether DNA viruses can augment gene expression of the human immunodeficiency virus (HIV), cotransfection experiments were carried out in which a recombinant plasmid containing the HIV long terminal repeat (LTR) linked to the chloramphenicol acetyltransferase (CAT) gene was transfected into cultured cells along with plasmids containing DNA from various distinct classes of DNA viruses. Molecular clones containing JC virus, BK virus, lymphotropic papovavirus, bovine papilloma virus, type 1 herpes simplex virus (HSV-1), and varicella-zoster virus sequences increased CAT expression directed by the HIV LTR. Trans-activation of the HIV LTR varied in different cell lines, but in each case the HIV tat gene product elicited the greatest stimulation. Primer-extension assays specific for HIV LTR mRNA revealed increased levels of steady-state RNA following transfection with HIV tat as well as with several of the DNA viruses. Virus-specific RNA expression paralleled the stimulation of CAT activity. More-than-additive effects were observed at both the RNA and protein levels when tat plus type 1 herpes simplex virus DNAs or tat plus JC virus DNAs were transfected into cells with the HIV LTR-CAT plasmid. These data suggest that coinfection of cells by HIV and some DNA viruses can stimulate the expression of HIV.

MeSH Terms

Antigens, Viral, Tumor/genetics Cell Line DNA Viruses/genetics Gene Expression Regulation HIV/genetics Humans Promoter Regions, Genetic RNA, Viral/genetics RNA-Directed DNA Polymerase/genetics Repetitive Sequences, Nucleic Acid Species Specificity Transcription, Genetic

Chemicals

Antigens, Viral, Tumor RNA, Viral RNA-Directed DNA Polymerase

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Gendelman H E

Phelps W

Feigenbaum L

Ostrove J M

Adachi A

Howley P M

Khoury G

Ginsberg H S

Martin M A

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.24.9759

Published

1986-12-00

Pages

9759-63

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC387220

Subset

External Links

PubMed source DOI: 10.1073/pnas.83.24.9759 PMC Full Text View journal details

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Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses.

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