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PMID: 3476953 Published · ppublish English Journal Article

Trans-activation of human immunodeficiency virus gene expression is mediated by nuclear events.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 18 ·1987-09-00 ·Pages 6364-8

Hauber J, Perkins A, Heimer EP, Cullen BR

Abstract

Human immunodeficiency virus encodes a gene product termed tat that is able to activate viral gene expression when present in trans. The mechanism of action of the tat gene product appears to be bimodal, resulting in both an increase in the steady-state level of viral mRNA and the enhanced translation of that RNA. In this report we have examined the mechanism by which tat elevates viral mRNA levels. Data are presented demonstrating that tat acts by increasing the rate of viral transcription, rather than by modulating the stability of viral mRNA. Indirect immunofluorescence was used to show that tat is predominantly localized in the nucleus of expressing cells, a location consistent with a role in the regulation of viral transcription. These results suggest that tat could play a role in human immunodeficiency virus replication essentially similar to that proposed for the trans-acting nuclear gene products described for several other virus species.

MeSH Terms
Cell Compartmentation Cell Nucleus/physiology DNA-Binding Proteins/physiology Gene Expression Regulation HIV/genetics Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Retroviridae Proteins/physiology Transcription Factors/genetics Transcription, Genetic
Chemicals
DNA-Binding Proteins RNA, Messenger RNA, Viral Retroviridae Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hauber J
Perkins A
Heimer E P
Cullen B R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.18.6364
Published
1987-09-00
Pages
6364-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299076
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.18.6364 PMC Full Text View journal details
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