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PMID: 2942759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Adenovirus E1B proteins are required for accumulation of late viral mRNA and for effects on cellular mRNA translation and transport.

Molecular and cellular biology ·Vol. 5 ·No. 10 ·1985-10-00 ·Pages 2552-8

Babiss LE, Ginsberg HS, Darnell JE

Abstract

Late in adenovirus infection, large amounts of viral mRNA accumulate while cell mRNA transport and translation decrease. Viruses deleted in the E1B region of type 5 adenovirus do not produce the same outcome: (i) viral mRNA synthesis by the mutants is normal, delivery to the cytoplasm is 50 to 75% of normal, but steady-state levels of viral mRNA are decreased 10-fold; (ii) cell mRNA synthesis and transport continue normally in the mutant virus-infected cell; and (iii) translation of preexisting cell mRNA which is disrupted in wild-type infection remains normal in mutant-virus-infected cells. Thus E1B proteins are required for accumulation of virus mRNA and for induction of the failure of host cell mRNA transport and translation. If a single function is involved, by inference the transport and some aspect of translation of mRNAs could be linked.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Biological Transport Capsid/biosynthesis,genetics Capsid Proteins Cell Nucleus/metabolism Cytoplasm/metabolism Gene Expression Regulation HeLa Cells Humans Oncogene Proteins, Viral/genetics Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/genetics,metabolism RNA, Viral/genetics
Chemicals
Adenovirus Early Proteins Capsid Proteins Oncogene Proteins, Viral RNA, Messenger RNA, Viral hexon capsid protein, Adenovirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Babiss L E
Ginsberg H S
Darnell J E
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28 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.10.2552-2558.1985
Published
1985-10-00
Pages
2552-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366989
Subset
IM
Grants
NIAID NIH HHS · AI12052 · United States
NCI NIH HHS · CA16006-12 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.10.2552-2558.1985 PMC Full Text View journal details
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