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PMID: 3181141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutational analysis of the 5' non-coding region of human immunodeficiency virus type 1: effects of secondary structure on translation.

The EMBO journal ·Vol. 7 ·No. 9 ·1988-09-00 ·Pages 2831-7

Parkin NT, Cohen EA, Darveau A, Rosen C, Haseltine W, Sonenberg N

Abstract

The first 111 nt from the 5' end of human immunodeficiency virus type 1 (HIV-1) mRNAs are shown to have a strong inhibitory effect on the translation of mRNA in in vitro translation extracts as well as in Xenopus oocytes. Mutations in the sequence of the 5' untranslated region (UTR) designed to disrupt predicted secondary structure of this region relieve the inhibition. Inhibition is restored by mutations that reconstruct the predicted secondary structure. The accessibility of the 5'-terminal cap structure was also found to be increased by some of these mutations. We conclude that secondary structure in the 5' UTR of HIV-1 mRNAs and resultant inaccessibility of the cap structure is responsible for the inhibition of translation. The implications of these findings for the understanding of the life cycle of HIV-1 are discussed.

MeSH Terms
Animals Chloramphenicol O-Acetyltransferase/biosynthesis,genetics Female HIV-1/genetics Humans Microinjections Mutation Nucleic Acid Conformation Oocytes Plasmids Protein Biosynthesis RNA, Messenger/analysis,genetics RNA, Viral/analysis,genetics Transcription, Genetic Xenopus laevis
Chemicals
RNA, Messenger RNA, Viral Chloramphenicol O-Acetyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Parkin N T
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Cohen E A
Darveau A
Rosen C
Haseltine W
Sonenberg N
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-09-00
Pages
2831-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457075
Subset
IM
Grants
NCI NIH HHS · CA42098 · United States
External Links
PubMed source PMC Full Text View journal details
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