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PMID: 2474830 Published · ppublish English Journal Article

Immunogenicity of peptide fusions to hepatitis B virus core antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 16 ·1989-08-00 ·Pages 6283-7

Stahl SJ, Murray K

Abstract

Several gene fusions have been constructed in which coding sequences for antigenic regions of the pre-S sequences of hepatitis B virus, hepatitis B surface antigen, and the envelope protein of human immunodeficiency virus were linked to the 3' end of that for the first 144 residues of hepatitis B core antigen. The sequences were expressed efficiently in Escherichia coli to give stable products that assembled to form particles morphologically similar to hepatitis B core antigen itself. The products exhibited the antigenic and immunogenic characteristics of both the hepatitis B core antigen epitopes and the epitopes carried by the additional sequences, thus illustrating the value of such proteins as immunological reagents and potential vaccines.

MeSH Terms
Amino Acid Sequence Antibody Specificity Cloning, Molecular Epitopes/genetics Escherichia coli/genetics Genes Genes, Viral Hepatitis B Core Antigens/analysis,genetics,immunology Hepatitis B virus/genetics Molecular Sequence Data Plasmids Recombinant Fusion Proteins/analysis
Chemicals
Epitopes Hepatitis B Core Antigens Recombinant Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stahl S J
Biogen S.A., Geneva, Switzerland.
Murray K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.16.6283
Published
1989-08-00
Pages
6283-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297822
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.16.6283 PMC Full Text View journal details
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