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PMID: 6492255 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Large surface proteins of hepatitis B virus containing the pre-s sequence.

Journal of virology ·Vol. 52 ·No. 2 ·1984-11-00 ·Pages 396-402

Heermann KH, Goldmann U, Schwartz W, Seyffarth T, Baumgarten H, Gerlich WH

Abstract

The sequence of hepatitis B virus DNA contains an open reading frame which codes for a not-yet-identified protein of at least 389 amino acids. Only the products starting at the third (GP33/GP36) or the fourth (P24/GP27) initiation signal have been characterized as components of the viral surface antigen. We found a larger protein, P39, and its glycosylated form, GP42, in hepatitis B virus particles and viral surface antigen filaments. Immunological cross-reactions showed that P39/GP42 is partially homologous to P24/GP27 and GP33/GP36. The unique portion of its sequence bound monoclonal antibodies which had been induced by immunization with hepatitis B virus particles. Proteolytic cleavage patterns and subtype-specific size differences suggested that the sequence of P39 starts with the first initiation signal of the open reading frame. Its amino-terminal part (pre-s coded) is exposed at the viral surface and, probably, is highly immunogenic. A model is presented of how the open reading frame for the viral envelope leads to defined amounts of three different proteins.

MeSH Terms
Antibodies, Monoclonal Base Sequence DNA, Viral/genetics Genes Genes, Viral Hepatitis B Surface Antigens/genetics,isolation & purification Hepatitis B virus/genetics Humans Molecular Weight RNA, Messenger/genetics
Chemicals
Antibodies, Monoclonal DNA, Viral Hepatitis B Surface Antigens RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Heermann K H
Goldmann U
Schwartz W
Seyffarth T
Baumgarten H
Gerlich W H
References (32)
32 references, click to expand
  1. Standardized detection of hepatitis B surface antigen: determination of its serum concentration in weight units per volume.
    Dev Biol Stand. 1975;30:78-87 PMID: 1204978
  2. Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1530-4 PMID: 266193
  3. The genome of hepatitis B virus.
    Annu Rev Microbiol. 1977;31:357-77 PMID: 334041
  4. Detection of a new antibody system reacting with Dane particles in hepatitis B virus infection.
    Br Med J. 1978 Oct 14;2(6144):1056-8 PMID: 81702
  5. The labelling of galactose residues in hepatitis B surface antigen glycoprotein.
    J Gen Virol. 1978 Dec;41(3):447-57 PMID: 745007
  6. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
    J Immunol. 1979 Oct;123(4):1548-50 PMID: 113458
  7. Selective detection of IgM-antibody against core antigen of the hepatitis B virus by a modified enzyme immune assay.
    J Med Virol. 1979;4(3):227-38 PMID: 395275
  8. Hepatitis B surface antigen polypeptides: artifactual bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis caused by aggregation.
    J Virol. 1980 Jul;35(1):20-3 PMID: 7411691
  9. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.
    Nature. 1979 Oct 25;281(5733):646-50 PMID: 399327
  10. Hepatitis B virus genes and their expression in E. coli.
    Nature. 1979 Dec 6;282(5739):575-9 PMID: 399329
  11. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
    Science. 1981 Mar 27;211(4489):1437-8 PMID: 6162199
  12. Isolation and characterization of the major protein and glycoprotein of hepatitis B surface antigen.
    J Biol Chem. 1981 Jul 10;256(13):6975-83 PMID: 7240257
  13. Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):177-81 PMID: 7017709
  14. Biology of hepatitis B virus.
    Science. 1981 Jul 24;213(4506):406-11 PMID: 6264599
  15. Evidence for the presence of repeating antigenic determinants in the major polypeptides derived from hepatitis B surface antigen.
    Virology. 1981 Oct 15;114(1):71-80 PMID: 6169198
  16. Specificity and localization of the hepatitis B virus-associated protein kinase.
    J Virol. 1982 Jun;42(3):761-6 PMID: 6808156
  17. endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4540-4 PMID: 6812050
  18. Variable protein composition of hepatitis B surface antigen from different donors.
    Virology. 1982 Dec;123(2):436-42 PMID: 6817511
  19. Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using concanavalin A and peroxidase: application to arenavirus and flavivirus proteins.
    Anal Biochem. 1982 Dec;127(2):389-94 PMID: 7165111
  20. Structural relationships between minor and major proteins of hepatitis B surface antigen.
    J Virol. 1983 May;46(2):626-8 PMID: 6842680
  21. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  22. A hepatitis B surface antigen polypeptide (P31) with the receptor for polymerized human as well as chimpanzee albumins.
    Gastroenterology. 1983 Aug;85(2):268-74 PMID: 6305755
  23. Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region.
    J Virol. 1983 Oct;48(1):271-80 PMID: 6310147
  24. Cellular immunity to the hepatitis B virion in acute hepatitis type B.
    Clin Exp Immunol. 1983 Sep;53(3):645-50 PMID: 6616959
  25. Signals regulating hepatitis B surface antigen transcription.
    Nature. 1983 Sep 22-28;305(5932):336-8 PMID: 6621688
  26. The nature of polypeptides larger in size than the major surface antigen components of hepatitis b and like viruses in ground squirrels, woodchucks, and ducks.
    Virology. 1983 Oct 15;130(1):76-90 PMID: 6636542
  27. Safety and potency aspects in the preparation of an experimental HBsAg vaccine.
    Dev Biol Stand. 1983;54:23-31 PMID: 6228464
  28. Characterization of pre-s gene products in hepatitis B surface antigen.
    Dev Biol Stand. 1983;54:33-43 PMID: 6653888
  29. Nucleotide sequence of a cloned duck hepatitis B virus genome: comparison with woodchuck and human hepatitis B virus sequences.
    J Virol. 1984 Mar;49(3):782-92 PMID: 6699938
  30. A polypeptide containing 55 amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins.
    Gastroenterology. 1984 May;86(5 Pt 1):910-8 PMID: 6323244
  31. Transcription of the hepatitis B surface antigen gene in cultured murine cells initiates within the presurface region.
    J Virol. 1984 May;50(2):563-71 PMID: 6200609
  32. Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus.
    Science. 1984 Apr 27;224(4647):392-5 PMID: 6200931
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.52.2.396-402.1984
Published
1984-11-00
Pages
396-402
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254539
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.52.2.396-402.1984 PMC Full Text View journal details
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