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PMID: 6200609 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of the hepatitis B surface antigen gene in cultured murine cells initiates within the presurface region.

Journal of virology ·Vol. 50 ·No. 2 ·1984-05-00 ·Pages 563-71

Standring DN, Rutter WJ, Varmus HE, Ganem D

Abstract

Cloned hepatitis B virus (HBV) DNA directs the synthesis of the viral surface antigen (HBsAg) when introduced into mouse L cells by DNA transformation. We have used recombinants between the Rous sarcoma virus long terminal repeat and subgenomic fragments of HBV DNA to localize regions of the HBV genome required for HBsAg expression. Examination of HBV-specific RNA from such transformants indicates that transcription initiates at three distinct sites (153, 163, and 183 nucleotides upstream from the translation initiation codon for mature HBsAg). Thus in these cells, a large segment of the presurface reading frame is not represented in HBsAg mRNA. The termination site of this RNA lies within the coding sequences for the viral core antigen, some 1,094 +/- 10 base pairs downstream from the TAA stop codon for HBsAg. Two additional open reading frames are present in the resultant unspliced HBsAg RNA.

MeSH Terms
Animals Base Sequence Genes Genes, Viral Hepatitis B Surface Antigens/genetics Hepatitis B virus/genetics L Cells/enzymology Mice Plasmids Poly A/genetics RNA/genetics RNA, Messenger Thymidine Kinase/deficiency,genetics Transcription, Genetic
Chemicals
Hepatitis B Surface Antigens RNA, Messenger Poly A RNA Thymidine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Standring D N
Rutter W J
Varmus H E
Ganem D
References (26)
26 references, click to expand
  1. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  3. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  4. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  5. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  6. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.
    Nature. 1979 Oct 25;281(5733):646-50 PMID: 399327
  7. Hepatitis B virus genes and their expression in E. coli.
    Nature. 1979 Dec 6;282(5739):575-9 PMID: 399329
  8. Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4549-53 PMID: 6933503
  9. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  10. Analysis of avian leukosis virus DNA and RNA in bursal tumours: viral gene expression is not required for maintenance of the tumor state.
    Cell. 1981 Feb;23(2):311-22 PMID: 6258797
  11. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  12. Biology of hepatitis B virus.
    Science. 1981 Jul 24;213(4506):406-11 PMID: 6264599
  13. Nucleotide sequence of a cloned woodchuck hepatitis virus genome: comparison with the hepatitis B virus sequence.
    J Virol. 1982 Jan;41(1):51-65 PMID: 7086958
  14. Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA.
    J Virol. 1982 Apr;42(1):100-5 PMID: 7086959
  15. Persistent infection of humans with hepatitis B virus: mechanisms and consequences.
    Rev Infect Dis. 1982 Sep-Oct;4(5):1026-47 PMID: 6293033
  16. Expression of the hepatitis B virus surface, core and E antigen genes by stable rat and mouse cell lines.
    J Mol Biol. 1982 Nov 25;162(1):43-67 PMID: 6296407
  17. Structural relationships between minor and major proteins of hepatitis B surface antigen.
    J Virol. 1983 May;46(2):626-8 PMID: 6842680
  18. Core and E antigen synthesis in rodent cells transformed with hepatitis B virus DNA is associated with greater than genome length viral messenger RNAs.
    J Mol Biol. 1983 Apr 25;165(4):683-99 PMID: 6854629
  19. A hepatitis B surface antigen polypeptide (P31) with the receptor for polymerized human as well as chimpanzee albumins.
    Gastroenterology. 1983 Aug;85(2):268-74 PMID: 6305755
  20. Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA.
    Cell. 1983 Jul;33(3):705-16 PMID: 6307525
  21. A previously unrecognized influenza B virus glycoprotein from a bicistronic mRNA that also encodes the viral neuraminidase.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4879-83 PMID: 6308656
  22. Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region.
    J Virol. 1983 Oct;48(1):271-80 PMID: 6310147
  23. Signals regulating hepatitis B surface antigen transcription.
    Nature. 1983 Sep 22-28;305(5932):336-8 PMID: 6621688
  24. Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.
    EMBO J. 1983;2(4):511-4 PMID: 6313342
  25. Transcription of hepatitis B virus by RNA polymerase II.
    Mol Cell Biol. 1983 Oct;3(10):1766-73 PMID: 6646122
  26. Hepatitis B virus encodes an RNA polymerase III transcript.
    Mol Cell Biol. 1983 Oct;3(10):1774-82 PMID: 6646123
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.2.563-571.1984
Published
1984-05-00
Pages
563-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255672
Subset
IM
Grants
NIAID NIH HHS · AI 19744 · United States
NIAID NIH HHS · AI/CA 18782 · United States
External Links
PubMed source DOI: 10.1128/JVI.50.2.563-571.1984 PMC Full Text View journal details
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