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PMID: 6170067 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4510-4

MacKay P, Pasek M, Magazin M, Kovacic RT, Allet B, Stahl S, Gilbert W, Schaller H, Bruce SA, Murray K

Abstract

Several plasmids have been constructed which direct the synthesis of hepatitis B virus surface antigens in Escherichia coli either as the native polypeptide or fused to other plasmid encoded polypeptides. When injected into rabbits, extracts from bacteria carrying some of these plasmids induced the synthesis of antibodies to the antigens even though the extracts did not give satisfactory positive results in radioimmunoassay for them. Either the NH2-terminal segment or the COOH-terminal segment of the surface antigens alone was sufficient to elicit the immune response, but antibodies against the two segments showed different specificities. The results emphasize the value of an in vivo assay for the presence of antigens in crude cell extracts and illustrate the feasibility of this type of screening with laboratory animals.

MeSH Terms
Antibody Specificity Cloning, Molecular/methods DNA, Recombinant Epitopes Escherichia coli/genetics Gene Expression Regulation Hepatitis B Surface Antigens/genetics,immunology
Chemicals
DNA, Recombinant Epitopes Hepatitis B Surface Antigens
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
MacKay P
Pasek M
Magazin M
Kovacic R T
Allet B
Stahl S
Gilbert W
Schaller H
Bruce S A
Murray K
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.7.4510
Published
1981-07-00
Pages
4510-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319821
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.7.4510 PMC Full Text View journal details
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