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PMID: 6251458 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3369-73

Talmadge K, Stahl S, Gilbert W

Abstract

We made a series of plasmids with unique Pst restriction sites within or near the DNA that encodes the penicillinase signal sequence. Inserted DNA can be read in all three frames both within and immediately after the signal sequence. We cloned Pst-terminated DNA copies of the structural information for rat proinsulin and preproinsulin into these plasmids, forming a large number of hybrid penicillinase (bacterial) and insulin (eukaryotic) signal sequences. We then compared the levels of insulin antigen in the Escherichia coli periplasm with those inside the cells. We conclude that either the bacterial or the eukaryotic signal is sufficient to transport rat insulin antigen into the periplasmic space.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Transport Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics Genetic Vectors Insulin/immunology,metabolism Insulin Secretion Penicillinase/genetics Plasmids Proinsulin/genetics Protein Precursors/genetics,metabolism Rats
Chemicals
DNA, Recombinant Insulin Protein Precursors preproinsulin Proinsulin DNA Restriction Enzymes CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific Penicillinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Talmadge K
Stahl S
Gilbert W
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3369
Published
1980-06-00
Pages
3369-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349617
Subset
IM
Databases
GENBANK
M10480
External Links
PubMed source DOI: 10.1073/pnas.77.6.3369 PMC Full Text View journal details
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