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PMID: 7045698 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synthesis and assembly of hepatitis B virus surface antigen particles in yeast.

Nature ·Vol. 298 ·No. 5872 ·1982-07-22 ·Pages 347-50

Valenzuela P, Medina A, Rutter WJ, Ammerer G, Hall BD

Abstract

The surface antigens of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5'-flanking region of yeast alcohol dehydrogenase I as a promotor to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

MeSH Terms

Alcohol Dehydrogenase Alcohol Oxidoreductases/genetics Chemical Phenomena Chemistry Cloning, Molecular DNA, Recombinant DNA, Viral Gene Expression Regulation Hepatitis B Antibodies/immunology Hepatitis B Surface Antigens/genetics,immunology Humans Operon Plasmids Saccharomyces cerevisiae/genetics Transcription, Genetic

Chemicals

DNA, Recombinant DNA, Viral Hepatitis B Antibodies Hepatitis B Surface Antigens Alcohol Oxidoreductases Alcohol Dehydrogenase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Valenzuela P

Medina A

Rutter W J

Ammerer G

Hall B D

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/298347a0

Published

1982-07-22

Pages

347-50

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

J02560

External Links

PubMed source DOI: 10.1038/298347a0 View journal details

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