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PMID: 2453021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A high-conductance anion channel in adult amphibian skeletal muscle.

Pflugers Archiv : European journal of physiology ·Vol. 410 ·No. 6 ·1987-12-00 ·Pages 632-40

Woll KH, Leibowitz MD, Neumcke B, Hille B

Abstract

Membrane patches were excised from enzymatically dissociated frog toe muscle. High-conductance anion channels could be induced in previously quiet patches by 20-120 s depolarizations beyond +20 mV and then studied in the potential range from -80 to +60 mV for a long time. From reversal potentials the estimated permeability ratios PCl/PNa and PCl/Pglucuronate were near 3.5 and 4, respectively. There were probably 5 or more conductance levels (substates) for a single channel, the most common in symmetrical 110 mM NaCl being 260 and 70 pS at 10 degrees C. Gating was complex, with rapid and slow events and several gating modes, including periods of rapid flickering. Channels closed reversibly at potentials more negative than -50 mV. The channel was blocked by application to the cytoplasmic face of tannic acid, gallic acid, and zinc but not of DIDS or 9-anthracene-carboxylic acid, and it was blocked by extracellular zinc.

MeSH Terms
Animals Anions Gallic Acid/pharmacology Hydrolyzable Tannins/pharmacology In Vitro Techniques Ion Channels/physiology Membrane Potentials Muscles/physiology Rana esculenta Rana pipiens Zinc/pharmacology
Chemicals
Anions Hydrolyzable Tannins Ion Channels Gallic Acid Zinc
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Woll K H
Department of Physiology and Biophysics, University of Washington, School of Medicine, Seattle 98195.
Leibowitz M D
Neumcke B
Hille B
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00581324
Published
1987-12-00
Pages
632-40
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
Grants
NINDS NIH HHS · NS08174 · United States
NCRR NIH HHS · RR00374 · United States
External Links
PubMed source DOI: 10.1007/BF00581324 View journal details
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