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PMID: 2415972 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Single anion-selective channels in basolateral membrane of a mammalian tight epithelium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 22 ·1985-11-00 ·Pages 7791-5

Hanrahan JW, Alles WP, Lewis SA

Abstract

Basolateral membrane chloride permeability of surface cells from rabbit urinary bladder epithelium was studied using the patch-clamp technique. Two types of anion-selective channel were observed. One channel type showed inward rectification and had a conductance of 64 pS at-50 mV when bathed symmetrically by saline solution containing 150 mM chloride; the other resembled high-conductance voltage-dependent anion channels (VDACs). Both channels had the selectivity sequence Cl-approximately equal to Br-approximately equal to I- approximately equal to SCN- approximately equal to NO3- greater than F- greater than acetate greater than gluconate greater than Na+ approximately equal to K+ and were sensitive to the anion exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. Basolateral chloride conductance in urinary bladder is apparently due to the 64 pS anion channel, which is active at physiological potentials. Imperfect selectivity of this channel against cations might also account for the low, but finite, sodium permeability of the basolateral membrane.

MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & derivatives,pharmacology Animals Cell Membrane Permeability Chlorides/metabolism Epithelium/metabolism In Vitro Techniques Ion Channels/physiology Kinetics Membrane Potentials Rabbits Urinary Bladder/metabolism
Chemicals
Chlorides Ion Channels 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hanrahan J W
Alles W P
Lewis S A
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.22.7791
Published
1985-11-00
Pages
7791-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391420
Subset
IM
Grants
NIADDK NIH HHS · AM33243 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.22.7791 PMC Full Text View journal details
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