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PMID: 2413212 Published · ppublish English Journal Article

Single Cl- channels in molluscan neurones: multiplicity of the conductance states.

The Journal of membrane biology ·Vol. 86 ·No. 1 ·1985-00-00 ·Pages 9-15

Geletyuk VI, Kazachenko VN

Abstract

Properties of the single Cl channels were studied in excised patches of surface membrane from molluscan neurones using single-channel recording technique. These channels are controlled by Ca2+ and K+ acting on cytoplasmic and outer membrane surfaces, respectively, and by the membrane potential. The channels display about 16 intermediate conductance sublevels, each of them being multiples of approximately 12.5 pS. The upper level of the channel conductance is about 200 pS. The channel behavior is consistent with an aggregation of channel-forming subunits into a cluster.

MeSH Terms
Animals Calcium/metabolism Chlorides/metabolism Electric Conductivity Ion Channels/drug effects,physiology Kinetics Lymnaea Membrane Potentials Neurons/physiology Potassium/pharmacology
Chemicals
Chlorides Ion Channels Potassium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Geletyuk V I
Kazachenko V N
References (22)
22 references, click to expand
  1. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
    Pflugers Arch. 1981 Aug;391(2):85-100 PMID: 6270629
  2. Action of some foreign cations and anions on the chloride permeability of frog muscle.
    J Physiol. 1967 Apr;189(3):445-60 PMID: 6040156
  3. A transient calcium-dependent chloride current in the immature Xenopus oocyte.
    J Physiol. 1983 Sep;342:309-25 PMID: 6313909
  4. The potential-dependent K+ channel in molluscan neurones is organized in a cluster of elementary channels.
    Biochim Biophys Acta. 1984 Jun 13;773(1):132-42 PMID: 6329278
  5. Serotonin and cyclic AMP close single K+ channels in Aplysia sensory neurones.
    Nature. 1982 Sep 30;299(5882):413-7 PMID: 6289122
  6. Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle.
    Biophys J. 1983 Aug;43(2):237-41 PMID: 6311302
  7. Single channel K+ currents from HeLa cells.
    J Membr Biol. 1983;74(1):41-9 PMID: 6306245
  8. Sodium and calcium channels in bovine chromaffin cells.
    J Physiol. 1982 Oct;331:599-635 PMID: 6296372
  9. Completely isolated neurons in the mollusc, Lymnaea stagnalis. A new objective for nerve cell biology investigation.
    Comp Biochem Physiol A Comp Physiol. 1974 Sep 1;49(1A):89-100 PMID: 4153848
  10. [Chemical composition of calcium channels of characean algal cells].
    Biofizika. 1980 May-Jun;25(3):537-42 PMID: 6249400
  11. Structural and functional evidence for multiple channel complexes in the outer membrane of Neurospora crassa mitochondria.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2243-7 PMID: 6300902
  12. Inward current channels activated by intracellular Ca in cultured cardiac cells.
    Nature. 1981 Dec 24;294(5843):752-4 PMID: 6275271
  13. Single Ca2+-activated nonselective cation channels in neuroblastoma.
    Nature. 1982 Mar 25;296(5855):357-9 PMID: 6278324
  14. Patch-clamp studies of slow potential-sensitive potassium channels in longitudinal smooth muscle cells of rabbit jejunum.
    J Physiol. 1983 Jul;340:469-86 PMID: 6310100
  15. Inhibition of acetylcholine responses by intracellular calcium in Lymnaea stagnalis neurones.
    J Physiol. 1982 Feb;323:1-19 PMID: 6284913
  16. Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channels.
    Nature. 1981 Dec 3;294(5840):464-6 PMID: 6273743
  17. Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA.
    Nature. 1983 Oct 27-Nov 2;305(5937):805-8 PMID: 6314144
  18. Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells.
    Nature. 1981 Dec 3;294(5840):462-4 PMID: 6273742
  19. Properties of single calcium-activated potassium channels in cultured rat muscle.
    J Physiol. 1982 Oct;331:211-30 PMID: 6296366
  20. A voltage-gated anion channel from the electric organ of Torpedo californica.
    J Biol Chem. 1979 Oct 25;254(20):10161-6 PMID: 489590
  21. Ionic permeability of K, Na, and Cl in potassium-depolarized nerve. Dependency on pH, cooperative effects, and action of tetrodotoxin.
    Biophys J. 1981 Sep;35(3):677-97 PMID: 7272457
  22. Elementary currents through Ca2+ channels in guinea pig myocytes.
    Pflugers Arch. 1983 Sep;398(4):284-97 PMID: 6314245
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01871605
Published
1985-00-00
Pages
9-15
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01871605 View journal details
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