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PMID: 6306245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Single channel K+ currents from HeLa cells.

The Journal of membrane biology ·Vol. 74 ·No. 1 ·1983-00-00 ·Pages 41-9

Sauvé R, Roy G, Payet D

Abstract

The extracellular patch-clamp technique was used in order to investigate the presence of ionic channels in HeLa cells, a well-known cultured cell type obtained from an epidermoid carcinoma of the cervix. Under Gigohm-seal conditions, discrete current jumps could be observed with patch electrodes containing KCl. These channels were found to be mainly permeable to K+ and showed multiple levels of conductance. From single-channel I-V curve measurements, a strong rectification effect, characterized by a large inward and no detectable outward current, was observed. For negative membrane potentials (0 to -90 mV), the measured current-voltage relationship was found to be mostly linear, corresponding to a single-channel conductance of 40 pS. An analysis of some selected time records has revealed in addition that the probability of the channel to be in the open state was a function of the KCl concentration in the patch pipette.

MeSH Terms
Electric Conductivity HeLa Cells Humans Ion Channels/metabolism Membrane Potentials Potassium/metabolism Potassium Chloride
Chemicals
Ion Channels Potassium Chloride Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sauvé R
Roy G
Payet D
References (24)
24 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870593
Published
1983-00-00
Pages
41-9
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01870593 View journal details
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