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PMID: 24124015 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Review

The centrality of RNA for engineering gene expression.

Biotechnology journal ·Vol. 8 ·No. 12 ·2013-12-00 ·Pages 1379-95

Chappell J, Takahashi MK, Meyer S, Loughrey D, Watters KE, Lucks J

Abstract

Synthetic biology holds promise as both a framework for rationally engineering biological systems and a way to revolutionize how we fundamentally understand them. Essential to realizing this promise is the development of strategies and tools to reliably and predictably control and characterize sophisticated patterns of gene expression. Here we review the role that RNA can play towards this goal and make a case for why this versatile, designable, and increasingly characterizable molecule is one of the most powerful substrates for engineering gene expression at our disposal. We discuss current natural and synthetic RNA regulators of gene expression acting at key points of control--transcription, mRNA degradation, and translation. We also consider RNA structural probing and computational RNA structure predication tools as a way to study RNA structure and ultimately function. Finally, we discuss how next-generation sequencing methods are being applied to the study of RNA and to the characterization of RNA's many properties throughout the cell.

Keywords
Gene regulation Next-generation sequencing Non-coding RNA RNA structure Synthetic biology
MeSH Terms
Gene Expression Regulation RNA Riboswitch Synthetic Biology
Chemicals
Riboswitch RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chappell James
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA.
Takahashi Melissa K
Meyer Sarai
Loughrey David
Watters Kyle E
Lucks Julius
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Article Info
Journal
Biotechnology journal
Abbr.
Biotechnol J
ISSN
1860-7314
DOI
10.1002/biot.201300018
Published
2013-12-00
Epub
2013-00-04
Pages
1379-95
Language
English
Region
Germany
NLM ID
101265833
PMCID
PMC4033574
Subset
IM
External Links
PubMed source DOI: 10.1002/biot.201300018 PMC Full Text View journal details
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