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PMID: 23023598 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

An adaptor from translational to transcriptional control enables predictable assembly of complex regulation.

Nature methods ·Vol. 9 ·No. 11 ·2012-11-00 ·Pages 1088-94

Liu CC, Qi L, Lucks JB, Segall-Shapiro TH, Wang D, Mutalik VK, Arkin AP

Abstract

Bacterial regulators of transcriptional elongation are versatile units for building custom genetic switches, as they control the expression of both coding and noncoding RNAs, act on multigene operons and can be predictably tethered into higher-order regulatory functions (a property called composability). Yet the less versatile bacterial regulators of translational initiation are substantially easier to engineer. To bypass this tradeoff, we have developed an adaptor that converts regulators of translational initiation into regulators of transcriptional elongation in Escherichia coli. We applied this adaptor to the construction of several transcriptional attenuators and activators, including a small molecule-triggered attenuator and a group of five mutually orthogonal riboregulators that we assembled into NOR gates of two, three or four RNA inputs. Continued application of our adaptor should produce large collections of transcriptional regulators whose inherent composability can facilitate the predictable engineering of complex synthetic circuits.

MeSH Terms
5' Untranslated Regions/genetics Base Sequence Escherichia coli/genetics,metabolism Gene Expression Regulation, Bacterial Peptide Chain Initiation, Translational/physiology Protein Sorting Signals/physiology Synthetic Biology/methods Transcription Elongation, Genetic/drug effects,physiology Transcription, Genetic
Chemicals
5' Untranslated Regions Protein Sorting Signals
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Liu Chang C
Department of Bioengineering, University of California at Berkeley, Berkeley, California, USA.
Qi Lei
Lucks Julius B
Segall-Shapiro Thomas H
Wang Denise
Mutalik Vivek K
Arkin Adam P
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.2184
Published
2012-11-00
Epub
2012-00-30
Pages
1088-94
Language
English
Region
United States
NLM ID
101215604
Subset
IM
External Links
PubMed source DOI: 10.1038/nmeth.2184 View journal details
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