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PMID: 17567606 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast.

Nucleic acids research ·Vol. 35 ·No. 12 ·2007-00-00 ·Pages 4179-85

Weigand JE, Suess B

Abstract

Splicing of pre-mRNA is a critical step in mRNA maturation and disturbances cause several genetic disorders. We apply the synthetic tetracycline (tc)-binding riboswitch to establish a gene expression system for conditional tc-dependent control of pre-mRNA splicing in yeast. Efficient regulation is obtained when the aptamer is inserted close to the 5'splice site (SS) with the consensus sequence of the SS located within the aptamer stem. Structural probing indicates limited spontaneous cleavage within this stem in the absence of the ligand. Addition of tc leads to tightening of the stem and the whole aptamer structure which probably prevents recognition of the 5'SS. Combination of more then one aptamer-regulated intron increases the extent of regulation leading to highly efficient conditional gene expression systems. Our findings highlight the potential of direct RNA-ligand interaction for regulation of gene expression.

MeSH Terms
Alternative Splicing Aptamers, Nucleotide/chemistry Ligands Molecular Sequence Data Nucleic Acid Conformation RNA Precursors/metabolism RNA Splice Sites RNA, Messenger/metabolism Saccharomyces cerevisiae/genetics Tetracycline/pharmacology
Chemicals
Aptamers, Nucleotide Ligands RNA Precursors RNA Splice Sites RNA, Messenger Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weigand Julia E
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5, 91058 Erlangen, Germany.
Suess Beatrix
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkm425
Published
2007-00-00
Epub
2007-00-12
Pages
4179-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1919484
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkm425 PMC Full Text View journal details
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