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PMID: 8065323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proteins binding to 5' untranslated region sites: a general mechanism for translational regulation of mRNAs in human and yeast cells.

Molecular and cellular biology ·Vol. 14 ·No. 9 ·1994-09-00 ·Pages 5898-909

Stripecke R, Oliveira CC, McCarthy JE, Hentze MW

Abstract

We demonstrate that a bacteriophage protein and a spliceosomal protein can be converted into eukaryotic translational repressor proteins. mRNAs with binding sites for the bacteriophage MS2 coat protein or the spliceosomal human U1A protein were expressed in human HeLa cells and yeast. The presence of the appropriate binding protein resulted in specific, dose-dependent translational repression when the binding sites were located in the 5' untranslated region (UTR) of the reporter mRNAs. Neither mRNA export from the nucleus to the cytoplasm nor mRNA stability was demonstrably affected by the binding proteins. The data thus reveal a general mechanism for translational regulation: formation of mRNA-protein complexes in the 5' UTR controls translation initiation by steric blockage of a sensitive step in the initiation pathway. Moreover, the findings establish the basis for novel strategies to study RNA-protein interactions in vivo and to clone RNA-binding proteins.

MeSH Terms
Base Sequence Biological Transport Cell Nucleus/metabolism Cytoplasm/metabolism Gene Expression Regulation Gene Expression Regulation, Fungal HeLa Cells Humans Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Protein Biosynthesis RNA, Messenger/metabolism RNA-Binding Proteins/genetics Repressor Proteins/genetics Saccharomyces cerevisiae
Chemicals
Oligodeoxyribonucleotides RNA, Messenger RNA-Binding Proteins Repressor Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stripecke R
Gene Expression Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Oliveira C C
McCarthy J E
Hentze M W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.9.5898-5909.1994
Published
1994-09-00
Pages
5898-909
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359116
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.9.5898-5909.1994 PMC Full Text View journal details
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