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PMID: 230904 Published · ppublish English Journal Article

Neurons of the rat hypothalamus reactive with antisera against endorphins, ACTH, MSH and beta-LPH.

Cell and tissue research ·Vol. 204 ·No. 1 ·1979-00-00 ·Pages 1-15

Bloch B, Bugnon C, Fellmann D, Lenys D, Gouget A

Abstract

In rat brains intraventricularly injected with colchicine, the same discrete neurons of the arcuate and ventromedial nuclei can be stained with antisera against alpha- and beta-endorphins, (1-24)ACTH, (17-39)ACTH, alpha- and beta-MSH, and beta-LPH, as demonstrated by comparative studies in consecutive serial sections. These neurons are strongly reactive with anti-(17-39)ACTH, anti-beta-endorphin, anti-alpha-MSH and anti-beta-MSH, and more faintly stained with anti-alpha-endorphin, anti-beta-LPH and anti-(1-24)ACTH. Exceptionally, neurons reactive with anti-(17-39)ACTH and anti-beta-endorphin are poorly stained or completely negative with anti-alpha-MSH and anti-beta-MSH. Immunoreactive fibers end in the lateral median eminence and in the arcuate nucleus proper, or form ascending pathways along the third ventricle. Comparative studies with other antisera or with the Falck and Hillarp technique show that these neurons differ from the elements producing LH-RH, somatostatin, neurophysin, oxytocin, vasopressin and dopamine. These results suggest that the same neurons of the rat hypothalamus synthesize several neuropeptides identical with or immunologically related to endorphins, ACTH, alpha-MSH and beta-LPH, probably arising from a common precursor molecule similar to that found in the corticotropic cells of the pituitary. These neuropeptides of a common cellular and molecular origin might be involved in basic processes of the central nervous system as neurotramsmitters or neuromodulators.

MeSH Terms
Adrenocorticotropic Hormone/metabolism Animals Antibody Specificity Dopamine/metabolism Endorphins/metabolism Fluorescent Antibody Technique Gonadotropin-Releasing Hormone/metabolism Hypothalamus/cytology,metabolism Melanocyte-Stimulating Hormones/metabolism Neurons/metabolism Oligopeptides/metabolism Rats beta-Lipotropin/metabolism
Chemicals
Endorphins Oligopeptides Gonadotropin-Releasing Hormone Adrenocorticotropic Hormone Melanocyte-Stimulating Hormones beta-Lipotropin Dopamine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bloch B
Bugnon C
Fellmann D
Lenys D
Gouget A
References (47)
47 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00235160
Published
1979-00-00
Pages
1-15
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00235160 View journal details
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