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PMID: 349568 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Neurons containing beta-endorphin in rat brain exist separately from those containing enkephalin: immunocytochemical studies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1591-5

Bloom F, Battenberg E, Rossier J, Ling N, Guillemin R

Abstract

Well-characterized antisera to porcine beta-endorphin were used to localize immunoreactive sites in cryostat sections of formaldehyde-fixed rat brain by indirect immunohistochemistry. Specificity was established by absorption of immune sera with synthetic peptide fragments. Specific immunoreactivity was localized to neuronal perikarya in the basal tuberal hypothalamus, and to varicose nerve fibers which were distributed to midline nuclear areas throughout the diencephalon and anterior pons. These patterns of reactivity were unaffected by preabsorption of the immune sera with millimolar concentrations of Met5- or Leu5-enkephalin or alpha-endorphin. The beta-endorphin immunoreactive structures were morphologically separate from those cells and fibers reported to react with antisera to the enkephalins. One anti-beta-endorphin serum gave additional immunoreactivity with myelinated axons in limbic cortical zones; when absorbed with purified rat myelin basic protein, only the specific patterns of immunoreactivity remained. Thus, discrete beta-endorphin-containing neuronal circuits exist in rat brain and are anatomically distinguishable from enkephalin-containing nerve cell and fiber pathways.

MeSH Terms
Animals Basal Ganglia/metabolism Brain Mapping Endorphins/metabolism Enkephalins/metabolism Hypothalamus/cytology,metabolism Immunologic Techniques Neurons/metabolism Paraventricular Hypothalamic Nucleus/metabolism Pons/metabolism Rats Substantia Gelatinosa/metabolism Thalamus/metabolism
Chemicals
Endorphins Enkephalins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bloom F
Battenberg E
Rossier J
Ling N
Guillemin R
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.3.1591
Published
1978-03-00
Pages
1591-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411520
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.3.1591 PMC Full Text View journal details
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