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PMID: 2247464 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Putative tyrosine kinases expressed in K-562 human leukemia cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 22 ·1990-11-00 ·Pages 8913-7

Partanen J, Mäkelä TP, Alitalo R, Lehväslaiho H, Alitalo K

Abstract

Tyrosine phosphorylation is important in the transmission of growth and differentiation signals; known tyrosine kinases include several oncoproteins and growth factor receptors. Interestingly, some differentiated cell types, such as erythrocytes and platelets contain high amounts of phosphotyrosine. We analyzed tyrosine kinases expressed in the K-562 chronic myelogenous leukemia cell line, which has a bipotential erythroid and megakaryoblastoid differentiation capacity. Analysis of 359 polymerase chain reaction-amplified cDNA clones led to the identification of 14 different tyrosine kinase-related sequences (JTK1-14). Two of the clones (JTK2 and JTK4) represent unusual members of the fibroblast growth factor receptor gene family, and the clones JTK5, JTK11, and JTK14 may also belong to the family of receptor tyrosine kinases but lack a close relationship to any known tyrosine kinase. Each of these different genes has its own characteristic expression pattern in K-562 cells and several other human tumor cell lines. In addition, the JTK11 and JTK14 mRNAs are induced during the megakaryoblastoid differentiation of K-562 cells. These tyrosine kinases may have a role in the differentiation of megakaryoblasts or in the physiology of platelets.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA/genetics Gene Expression Regulation, Enzymologic Humans Leukemia, Myeloid/enzymology,genetics Molecular Sequence Data Multigene Family Phylogeny Polymerase Chain Reaction Protein-Tyrosine Kinases/genetics RNA, Messenger/genetics Sequence Homology, Nucleic Acid Tumor Cells, Cultured
Chemicals
RNA, Messenger DNA Protein-Tyrosine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Partanen J
Department of Virology and Pathology, University of Helsinki, Finland.
Mäkelä T P
Alitalo R
Lehväslaiho H
Alitalo K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.22.8913
Published
1990-11-00
Pages
8913-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55070
Subset
IM
Databases
GENBANK
M37781, M37782, M59373, M59374
External Links
PubMed source DOI: 10.1073/pnas.87.22.8913 PMC Full Text View journal details
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