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PMID: 3211147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human FGF-5 oncogene encodes a novel protein related to fibroblast growth factors.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 3487-95

Zhan X, Bates B, Hu XG, Goldfarb M

Abstract

We previously described the isolation of a human oncogene which had acquired transforming potential by a DNA rearrangement accompanying transfection of NIH 3T3 cells with human tumor DNA (X. Zhan, A. Culpepper, M. Reddy, J. Loveless, and M. Goldfarb, Oncogene 1:369-376, 1987). We now term this oncogene the FGF-5 gene, since it specifies the fifth documented protein related to fibroblast growth factors (FGFs. Two regions of the FGF-5 sequence, containing 122 of its 267 amino acid residues, were 40 to 50% homologous to the sequences of acidic and basic FGFs as well as to the sequences of the FGF-related oncoproteins int-2 and hst/KS3. The FGF-5 gene bears the three exon structures typical for members of this family. FGF-5 was found to be expressed in the neonatal brain and in 3 of the 13 human tumor cell lines examined. Several experiments strongly suggested that FGF-5 is a growth factor with properties common to those of acidic and basic FGFs. The rearrangement which activated the FGF-5 gene during DNA transfection had juxtaposed a retrovirus transcriptional enhancer just upstream from the native promoter of the gene.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cell Transformation, Neoplastic Cells, Cultured Fibroblast Growth Factors/genetics,isolation & purification Genes Humans Mice Mice, Inbred BALB C Mice, Inbred Strains Molecular Sequence Data Neoplasm Proteins/genetics Oncogenes Recombinant Fusion Proteins/isolation & purification
Chemicals
Neoplasm Proteins Recombinant Fusion Proteins Fibroblast Growth Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhan X
Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Bates B
Hu X G
Goldfarb M
References (38)
38 references, click to expand
  1. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:457-74 PMID: 1057474
  2. Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor.
    Science. 1984 Mar 23;223(4642):1296-9 PMID: 6199844
  3. Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
    J Natl Cancer Inst. 1977 Feb;58(2):209-14 PMID: 833871
  4. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
    J Natl Cancer Inst. 1977 Jul;59(1):221-6 PMID: 327080
  5. Induction of DNA synthesis in BALB/c 3T3 cells by serum components: reevaluation of the commitment process.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4481-5 PMID: 270695
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.
    Natl Cancer Inst Monogr. 1978 Dec;(49):5-9 PMID: 571047
  8. Translocation of proteins across membranes: the signal hypothesis and beyond.
    Symp Soc Exp Biol. 1979;33:9-36 PMID: 524275
  9. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  10. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  11. Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain.
    Science. 1984 Aug 31;225(4665):932-5 PMID: 6382607
  12. The isolation and purification of two anionic endothelial cell growth factors from human brain.
    Biochem Biophys Res Commun. 1984 Oct 15;124(1):262-8 PMID: 6388573
  13. Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cells.
    Nature. 1984 Nov 29-Dec 5;312(5993):418-23 PMID: 6095102
  14. Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6963-7 PMID: 6594674
  15. Pure brain-derived acidic fibroblast growth factor is a potent angiogenic vascular endothelial cell mitogen with sequence homology to interleukin 1.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6409-13 PMID: 2413439
  16. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6507-11 PMID: 3863109
  17. Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies.
    Science. 1985 Dec 20;230(4732):1385-8 PMID: 4071057
  18. Induction of angiogenesis by bovine brain derived class 1 heparin-binding growth factor.
    Biochemistry. 1985 Sep 10;24(19):4969-73 PMID: 2416338
  19. Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2448-52 PMID: 3458207
  20. Sequence, topography and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammary tumour virus.
    EMBO J. 1986 May;5(5):919-24 PMID: 3013624
  21. Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization.
    Science. 1986 Aug 1;233(4763):541-5 PMID: 3523756
  22. Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.
    Science. 1986 Aug 1;233(4763):545-8 PMID: 2425435
  23. Analysis of retroviral pol gene products with antisera raised against fusion proteins produced in Escherichia coli.
    J Virol. 1986 Aug;59(2):328-40 PMID: 2426463
  24. Basic fibroblast growth factor supports the survival of cerebral cortical neurons in primary culture.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7537-41 PMID: 3463983
  25. Human basic fibroblast growth factor: nucleotide sequence and genomic organization.
    EMBO J. 1986 Oct;5(10):2523-8 PMID: 3780670
  26. Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media.
    Mol Cell Biol. 1986 Oct;6(10):3541-4 PMID: 3796592
  27. Capillary endothelial cells express basic fibroblast growth factor, a mitogen that promotes their own growth.
    Nature. 1987 Jan 15-21;325(6101):257-9 PMID: 2433585
  28. Fibroblast growth factors are present in the extracellular matrix produced by endothelial cells in vitro: implications for a role of heparinase-like enzymes in the neovascular response.
    Biochem Biophys Res Commun. 1987 Jan 30;142(2):428-35 PMID: 2434094
  29. Potential oncogene product related to growth factors.
    Nature. 1987 Apr 30-May 6;326(6116):833 PMID: 3574458
  30. cDNA sequence of human transforming gene hst and identification of the coding sequence required for transforming activity.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2980-4 PMID: 2953031
  31. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family.
    Cell. 1987 Aug 28;50(5):729-37 PMID: 2957062
  32. Human vascular smooth muscle cells both express and respond to heparin-binding growth factor I (endothelial cell growth factor).
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7124-8 PMID: 2444975
  33. Genomic sequence of hst, a transforming gene encoding a protein homologous to fibroblast growth factors and the int-2-encoded protein.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7305-9 PMID: 2959959
  34. Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes.
    Mol Cell Biol. 1987 Oct;7(10):3438-45 PMID: 3683388
  35. Human oncogenes detected by a defined medium culture assay.
    Oncogene. 1987;1(4):369-76 PMID: 3330780
  36. Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7 PMID: 6292901
  37. Purification and characterization of acidic fibroblast growth factor from bovine brain.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):357-61 PMID: 6582493
  38. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4120-4 PMID: 1069301
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.3487-3495.1988
Published
1988-08-00
Pages
3487-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363586
Subset
IM
Databases
GENBANK
M21617, M23534, M23535, M23536
External Links
PubMed source DOI: 10.1128/mcb.8.8.3487-3495.1988 PMC Full Text View journal details
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