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PMID: 22390342 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Cross-linking measurements of the Potato leafroll virus reveal protein interaction topologies required for virion stability, aphid transmission, and virus-plant interactions.

Journal of proteome research ·Vol. 11 ·No. 5 ·2012-05-04 ·Pages 2968-81

Chavez JD, Cilia M, Weisbrod CR, Ju HJ, Eng JK, Gray SM, Bruce JE

Abstract

Protein interactions are critical determinants of insect transmission for viruses in the family Luteoviridae. Two luteovirid structural proteins, the capsid protein (CP) and the readthrough protein (RTP), contain multiple functional domains that regulate virus transmission. There is no structural information available for these economically important viruses. We used Protein Interaction Reporter (PIR) technology, a strategy that uses chemical cross-linking and high resolution mass spectrometry, to discover topological features of the Potato leafroll virus (PLRV) CP and RTP that are required for the diverse biological functions of PLRV virions. Four cross-linked sites were repeatedly detected, one linking CP monomers, two within the RTP, and one linking the RTP and CP. Virus mutants with triple amino acid deletions immediately adjacent to or encompassing the cross-linked sites were defective in virion stability, RTP incorporation into the capsid, and aphid transmission. Plants infected with a new, infectious PLRV mutant lacking 26 amino acids encompassing a cross-linked site in the RTP exhibited a delay in the appearance of systemic infection symptoms. PIR technology provided the first structural insights into luteoviruses which are crucially lacking and are involved in vector-virus and plant-virus interactions. These are the first cross-linking measurements on any infectious, insect-transmitted virus.

MeSH Terms
Agrobacterium tumefaciens/genetics,metabolism Amino Acid Sequence Animals Aphids/metabolism,virology Binding Sites Capsid Proteins/genetics,metabolism Feeding Behavior Host-Pathogen Interactions Insect Vectors/virology Luteoviridae/isolation & purification,pathogenicity,physiology Mass Spectrometry Molecular Sequence Data Plant Diseases/virology Plasmids/genetics,metabolism Protein Interaction Mapping/methods Solanum tuberosum/virology Tobacco/virology Virus Assembly
Chemicals
Capsid Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chavez Juan D
Department of Genome Sciences, University of Washington, Seattle, Washington 98109, USA.
Cilia Michelle
Weisbrod Chad R
Ju Ho-Jong
Eng Jimmy K
Gray Stewart M
Bruce James E
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Article Info
Journal
Journal of proteome research
Abbr.
J Proteome Res
ISSN
1535-3907
DOI
10.1021/pr300041t
Published
2012-05-04
Epub
2012-00-30
Pages
2968-81
Language
English
Region
United States
NLM ID
101128775
PMCID
PMC3402239
Subset
IM
Grants
NCRR NIH HHS · S10 RR025107-02 · United States
NCRR NIH HHS · 7S10RR025107 · United States
NCRR NIH HHS · R01 RR023334 · United States
NIGMS NIH HHS · R01 GM097112-01 · United States
NCRR NIH HHS · S10 RR025107 · United States
NIGMS NIH HHS · R01 GM086688-01 · United States
NIGMS NIH HHS · 2R01GM086688-03 · United States
NIGMS NIH HHS · R01 GM097112 · United States
NCRR NIH HHS · 5R01RR023334-04 · United States
NIGMS NIH HHS · R01 GM086688 · United States
NCRR NIH HHS · R01 RR023334-04 · United States
External Links
PubMed source DOI: 10.1021/pr300041t PMC Full Text View journal details
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